Grimm-Jørgensen Y
Brain Res. 1987 Feb 10;403(1):121-6. doi: 10.1016/0006-8993(87)90130-2.
Isolated neurons from the gastropod Physella heterostropha fail to regenerate their neurites if cultured in defined medium in the absence of growth factors. A significant number of cultured neurons extend neurites if they are cultured in brain-conditioned or hemocyte-conditioned medium. Synthetic somatostatin and salmon calcitonin also stimulate neurite regeneration in a dose-dependent manner. Thyrotropin-releasing hormone, arginine vasotocin, and eledoisin fail to promote neurite outgrowth. It is concluded that, like other molluscan neurons, isolated neurons from Physella require the presence of factors that are released by brain tissue or are present in the hemolymph, and that somatostatin and calcitonin can, in part, substitute for the endogenous neurite-regenerating activities.
如果在没有生长因子的特定培养基中培养，来自腹足纲动物异形膀胱螺的分离神经元无法再生其神经突。如果在脑条件培养基或血细胞条件培养基中培养，大量培养的神经元会伸出神经突。合成的生长抑素和鲑鱼降钙素也以剂量依赖的方式刺激神经突再生。促甲状腺激素释放激素、精氨酸加压催产素和eledoisin不能促进神经突生长。得出的结论是，与其他软体动物神经元一样，来自异形膀胱螺的分离神经元需要脑组织释放的因子或血淋巴中存在的因子，并且生长抑素和降钙素可以部分替代内源性神经突再生活性。
