Ahn Hyo-Suk, Lee Dong-Hyeon, Kim Tae-Jung, Shin Hyeon-Cheol, Jeon Hui-Kyung
1 Division of Cardiology, Department of Internal Medicine, The Catholic University of Korea, Uijeongbu St. Mary's Hospital , Uijeongbu, Korea.
2 Division of Cardiology, Department of Internal Medicine, The Catholic University of Korea, Seoul St. Mary's Hospital , Seoul, Korea.
J Med Food. 2017 Oct;20(10):944-950. doi: 10.1089/jmf.2017.3919. Epub 2017 Aug 17.
Long-term therapy with doxorubicin (DOX) is associated with high incidence of cumulative and irreversible dilated cardiomyopathy. The goal of this study was to evaluate the cardioprotective effects and safety of a phlorotannin extract from a brown algae Ecklonia cava (Seapolynol™, SPN) against DOX-induced cardiotoxicity in a rat model. A total of 42 rats were divided into six groups: control, low-dose SPN (LDS), high-dose SPN (HDS), DOX, DOX with low-dose SPN (DOX+LDS), and DOX with high-dose SPN (DOX+HDS). Echocardiography was performed at baseline and after 6 weeks. In left ventricular (LV) ejection fraction, DOX and DOX+LDS groups showed significant decreases (P < .001), while LDS, HDS, and DOX+HDS groups showed no significant change compared with control group. In LV mass index, DOX and DOX+LDS groups showed significant increases (P < .001 and P = .013), while LDS, HDS, and DOX+HDS groups showed no significant change compared with control group. In electron microscopy of the LV wall tissue, DOX+HDS group showed markedly less impaired myofibrils and mitochondria compared with both DOX and DOX+LDS groups. On the findings in echocardiography and electron microscopy, 6-week oral administration of SPN was safe and cardioprotective in a DOX-induced rat cardiotoxicity model in a dose-dependent manner.
长期使用阿霉素（DOX）治疗会导致累积性和不可逆性扩张型心肌病的高发病率。本研究的目的是评估一种从褐藻海蕴中提取的间苯三酚单宁（Seapolynol™，SPN）对大鼠模型中DOX诱导的心脏毒性的心脏保护作用和安全性。总共42只大鼠被分为六组：对照组、低剂量SPN组（LDS）、高剂量SPN组（HDS）、DOX组、DOX与低剂量SPN联合组（DOX+LDS）以及DOX与高剂量SPN联合组（DOX+HDS）。在基线和6周后进行超声心动图检查。在左心室（LV）射血分数方面，DOX组和DOX+LDS组显著降低（P < 0.001），而LDS组、HDS组和DOX+HDS组与对照组相比无显著变化。在LV质量指数方面，DOX组和DOX+LDS组显著增加（P < 0.001和P = 0.013），而LDS组、HDS组和DOX+HDS组与对照组相比无显著变化。在LV壁组织的电子显微镜检查中，与DOX组和DOX+LDS组相比，DOX+HDS组的肌原纤维和线粒体受损明显较少。基于超声心动图和电子显微镜的结果，在DOX诱导的大鼠心脏毒性模型中，6周口服SPN以剂量依赖的方式具有安全性和心脏保护作用。
