Clinical Nutrition Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah, Saudi Arabia.
Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Open Vet J. 2024 Jul;14(7):1668-1676. doi: 10.5455/OVJ.2024.v14.i7.16. Epub 2024 Jul 31.
Doxorubicin (DOX) is a chemotherapeutic drug applied clinically for the remedy of cancer, but its possibly life-threatening cardiotoxicity effects remain a concern.
After that, this study evaluates the cardioprotective impacts of (LSS) oil on DOX induced cardiomyopathy in rats.
Wistar male rats ( = 28, weighting 190-210 g) were arbitrarily allocated into four equal groups. Group 1 control group (CTR) received normal saline orally (1 ml/kg); group 2 (DOX) received DOX (10 mg/kg); group 3 (DOLS) received DOX + 3 g of seeds oil/kg; group 4 (LSSO) received LSSO (3 g/kg) daily for 18 days. The serum samples were collected to determine the creatine kinase-MB (CK-MB) isoenzyme, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), and Troponin I activity. At the same time, the catalase, malondialdehyde (MDA), and reduced glutathione (GSH) were assessed in heart tissues. Additionally, histopathological investigations for the heart tissue were performed.
Results revealed no significant change in CK-MB levels between the DOLS group compared to the CTR group ( > 0.05). DOX group confirmed a substantial increase in AST, LDH, and Troponin1 serum levels compared to DOLS and LLSO groups ( < 0.05). The study demonstrated the antioxidant activity of LSS oil against DOX-induced toxicity. The DOX group significantly reduced GSH and catalase levels, with an increase in MDA levels compared to DOLS and LLSO groups. Histopathological analysis showed protective properties of LSS oil against myocardial damage caused by DOX.
This study highlights the favorable impacts of LSS oil in mitigating DOX-triggered cardiotoxicity in a rat model.
多柔比星（DOX）是一种临床上用于治疗癌症的化疗药物，但它可能危及生命的心脏毒性作用仍然令人担忧。
本研究评估了亚麻籽油（LSS）对 DOX 诱导的大鼠心肌病的心脏保护作用。
将 28 只雄性 Wistar 大鼠（体重 190-210 g）随机分为四组。第 1 组为对照组（CTR），口服生理盐水（1 ml/kg）；第 2 组（DOX）给予 DOX（10 mg/kg）；第 3 组（DOLS）给予 DOX+3 g 亚麻籽油/kg；第 4 组（LSSO）给予 LSSO（3 g/kg），每天 1 次，共 18 天。收集血清样本，测定肌酸激酶同工酶（CK-MB）、乳酸脱氢酶（LDH）、天门冬氨酸氨基转移酶（AST）和肌钙蛋白 I 活性。同时测定心脏组织中的过氧化氢酶、丙二醛（MDA）和还原型谷胱甘肽（GSH）。此外，还对心脏组织进行了组织病理学检查。
与对照组相比，DOLS 组 CK-MB 水平无显著变化（ > 0.05）。与 DOLS 和 LSSO 组相比，DOX 组 AST、LDH 和肌钙蛋白 1 血清水平显著升高（ < 0.05）。研究表明，LSS 油具有抗氧化活性，可对抗 DOX 诱导的毒性。与 DOLS 和 LSSO 组相比，DOX 组 GSH 和过氧化氢酶水平显著降低，MDA 水平升高。组织病理学分析显示，LSS 油对 DOX 引起的心肌损伤具有保护作用。
本研究强调了 LSS 油减轻 DOX 诱导的大鼠心肌毒性的有利影响。
