Gonzalez-Guijarro L, Lopez-Ruiz M P, Bodegas G, Prieto J C, Arilla E
Exp Mol Pathol. 1987 Apr;46(2):153-8. doi: 10.1016/0014-4800(87)90061-x.
Administration of cysteamine in rabbits elicited a rapid depletion of both duodenal mucosa and plasma somatostatin. A significant reduction was observed within 5 min, returning toward control values by 150 min. The depletion of somatostatin was associated with an increase in the binding capacity and a decrease in the affinity of both high- and low-affinity binding sites present in cytosol of duodenal mucosa. Incubation of cytosolic fraction from control rabbits with 1 mM cysteamine did not modify somatostatin binding. Furthermore, addition of cysteamine at the time of binding assay did not affect the integrity of 125I-Tyr11-somatostatin. It is concluded that in vivo administration of cysteamine to rabbits depletes both duodenal mucosa and plasma somatostatin and leads to up-regulation of duodenal somatostatin binding sites.
给兔子注射半胱胺会导致十二指肠黏膜和血浆中生长抑素迅速耗竭。5分钟内观察到显著减少，150分钟时恢复至对照值。生长抑素的耗竭与十二指肠黏膜胞质溶胶中高亲和力和低亲和力结合位点的结合能力增加及亲和力降低有关。用1 mM半胱胺孵育对照兔的胞质组分不会改变生长抑素结合。此外，在结合测定时添加半胱胺不会影响125I-Tyr11-生长抑素的完整性。结论是，给兔子体内注射半胱胺会耗尽十二指肠黏膜和血浆中的生长抑素，并导致十二指肠生长抑素结合位点上调。
