Reddy S, Bibby N J, Fisher S L, Elliott R B
Gen Comp Endocrinol. 1986 Oct;64(1):157-62. doi: 10.1016/0016-6480(86)90042-0.
Antibodies to insulin, glucagon, pancreatic polypeptide (PP), and somatostatin were used in the immunofluorescence procedure to demonstrate localization of the four hormones in cells of the pancreatic islets of the brushtailed possum, Trichosurus vulpecula. Most pancreatic islets revealed some differences in the topographical distribution and cell number of each endocrine cell type. Insulin immunoreactive cells were observed in most islets where they occurred as groups of cells peripherally and within the islet. In several islets glucagon cells were the predominant cell population and were distributed peripherally as well as centrally. Pancreatic polypeptide cells were fewer in number and usually occurred as single cells within the islet. Cells immunoreactive to antisomatostatin serum were observed in varying numbers in the peripheral and central regions of the islet. The present immunofluorescence study demonstrates differences in the topographical distribution of the four major pancreatic hormones between a marsupial species and several of the eutherian mammals.
在免疫荧光实验中，使用了抗胰岛素、胰高血糖素、胰多肽（PP）和生长抑素的抗体，以证明这四种激素在帚尾袋貂（Trichosurus vulpecula）胰岛细胞中的定位。大多数胰岛显示出每种内分泌细胞类型在地形分布和细胞数量上存在一些差异。在大多数胰岛中观察到胰岛素免疫反应性细胞，它们以细胞群的形式出现在胰岛周边和内部。在几个胰岛中，胰高血糖素细胞是主要的细胞群体，分布在周边和中央。胰多肽细胞数量较少，通常以单个细胞的形式出现在胰岛内。在胰岛的周边和中央区域观察到不同数量的对抗生长抑素血清有免疫反应的细胞。目前的免疫荧光研究表明，有袋类动物与几种真兽类哺乳动物之间，四种主要胰腺激素的地形分布存在差异。
