Harvard Medical School, Boston, MA, USA.
TRANSCEND Research, Department of Neurology, Massachusetts General Hospital, Charlestown, Boston, MA 02129, USA.
J Diabetes Res. 2017;2017:4124967. doi: 10.1155/2017/4124967. Epub 2017 Jul 27.
Dietary management of autoimmune diabetes includes low glycemic foods classified from the glycemic index, but it does not consider the role that immunoreactive foods may play with the immunological etiology of the disease. We measured the reactivity of either monoclonal or polyclonal affinity-purified antibodies to insulin, insulin receptor alpha, insulin receptor beta, zinc transporter 8 (ZnT8), tyrosine phosphatase-based islet antigen 2 (IA2), and glutamic acid decarboxylase (GAD) 65 and 67 against 204 dietary proteins that are commonly consumed. Dietary protein determinants included unmodified (raw) and modified (cooked and roasted) foods, herbs, spices, food gums, brewed beverages, and additives. There was no immune reactivity between insulin or insulin receptor beta and dietary proteins. However, we identified strong to moderate immunological reactivity with antibodies against insulin receptor alpha, ZnT8, IA2, GAD-65, and GAD-67 with several dietary proteins. We also identified 49 dietary proteins found in foods classified as low glycemic foods with immune reactivity to autoimmune target sites. Laboratory analysis of immunological cross-reactivity between pancreas target sites and dietary proteins is the initial step necessary in determining whether dietary proteins may play a potential immunoreactive role in autoimmune diabetes.
自身免疫性糖尿病的饮食管理包括根据血糖生成指数分类的低血糖食物，但它没有考虑到免疫反应性食物可能在疾病的免疫学病因中所起的作用。我们测量了单克隆或多克隆亲和纯化的针对胰岛素、胰岛素受体α、胰岛素受体β、锌转运蛋白 8（ZnT8）、基于酪氨酸磷酸酶的胰岛抗原 2（IA2）和谷氨酸脱羧酶（GAD）65 和 67 的抗体对 204 种常见食用的膳食蛋白的反应性。膳食蛋白决定因素包括未修饰（生）和修饰（烹饪和烤制）食物、草药、香料、食品胶、酿造饮料和添加剂。胰岛素或胰岛素受体β与膳食蛋白之间没有免疫反应性。然而，我们发现针对胰岛素受体α、ZnT8、IA2、GAD-65 和 GAD-67 的抗体与几种膳食蛋白具有强烈至中度的免疫反应性。我们还发现了 49 种存在于低血糖食物中的膳食蛋白，它们与自身免疫靶位具有免疫反应性。在确定膳食蛋白是否可能在自身免疫性糖尿病中发挥潜在的免疫反应性作用之前，对胰腺靶位和膳食蛋白之间的免疫交叉反应性进行实验室分析是必要的第一步。
