W. M. Keck FTMS Laboratory for Human Health Research, Department of Chemistry, North Carolina State University, Raleigh, NC, 27695, USA.
Department of Chemistry, North Carolina State University, Raleigh, NC, 27695, USA.
Anal Bioanal Chem. 2018 Jan;410(3):953-962. doi: 10.1007/s00216-017-0570-9. Epub 2017 Aug 17.
Terpenes are the largest class of natural products with a wide range of applications including use as pharmaceuticals, fragrances, flavorings, and agricultural products. Terpenes are biosynthesized by the condensation of a variable number of isoprene units resulting in linear polyisoprene diphosphate units, which can then be cyclized by terpene synthases into a range of complex structures. While these cyclic structures have immense diversity and potential in different applications, their direct analysis in biological buffer systems requires intensive sample preparation steps such as salt cleanup, extraction with organic solvents, and chromatographic separations. Electrospray post-ionization can be used to circumvent many sample cleanup and desalting steps. SESI and IR-MALDESI are two examples of ionization methods that employ electrospray post-ionization at atmospheric pressure and temperature. By coupling the two techniques and doping the electrospray solvent with silver ions, olefinic terpenes of different classes and varying degrees of volatility were directly analyzed from a biological buffer system with no sample workup steps.
萜类化合物是最大的天然产物类别，具有广泛的应用，包括用作药物、香料、调味剂和农产品。萜类化合物是由异戊二烯单元的可变数量缩合而成的，产生线性聚异戊二烯二磷酸酯单元，然后萜烯合酶可以将其环化，形成一系列复杂的结构。虽然这些环状结构在不同的应用中具有巨大的多样性和潜力，但在生物缓冲体系中直接分析需要进行密集的样品制备步骤，如盐清除、有机溶剂提取和色谱分离。电喷雾后电离可用于避免许多样品净化和脱盐步骤。SESI 和 IR-MALDESI 是两种在大气压和温度下采用电喷雾后电离的电离方法的示例。通过将这两种技术结合起来，并在电喷雾溶剂中掺杂银离子，可以直接从生物缓冲体系中分析不同类别和挥发性程度不同的烯烃萜类化合物，而无需进行样品制备步骤。
