Harrelson A L, Rostene W, McEwen B S
J Neurochem. 1987 May;48(5):1648-55. doi: 10.1111/j.1471-4159.1987.tb05714.x.
Glucocorticoid hormones are known to affect limbic system structures that have high levels of specific receptors for glucocorticoids, especially the hippocampus (HIPP). To understand how glucocorticoids may affect synaptic transmission, we have tested the effects of adrenal removal and glucocorticoid replacement on neurotransmitter-stimulated cyclic AMP accumulation in brain slices from the rat limbic system. Adrenalectomy (ADX) caused an enhancement of vasoactive intestinal peptide (VIP)-stimulated cyclic AMP accumulation in HIPP, amygdala (AMYG), and septum (SEP). In HIPP, ADX increased the cyclic AMP response to isoproterenol (ISOP) and decreased the response to histamine (HIST). In the AMYG and SEP, ADX did not affect significantly the action of ISOP, but ADX did decrease the response to HIST in AMYG. Administration of dexamethasone or corticosterone reversed the effects of ADX on cyclic AMP accumulation in the HIPP. The dexamethasone action on VIP-stimulated cyclic AMP accumulation takes place within 48 h and is most apparent in the mid-range of the VIP dose-response curve. These results demonstrate that glucocorticoids regulate neurotransmitter-stimulated cyclic AMP generation in a fashion that is specific, both for the neurotransmitter involved and for the brain regions affected.
已知糖皮质激素会影响边缘系统结构，这些结构具有高水平的糖皮质激素特异性受体，尤其是海马体（HIPP）。为了了解糖皮质激素如何影响突触传递，我们测试了肾上腺切除和糖皮质激素替代对大鼠边缘系统脑片神经递质刺激的环磷酸腺苷（cAMP）积累的影响。肾上腺切除术（ADX）导致海马体、杏仁核（AMYG）和隔区（SEP）中血管活性肠肽（VIP）刺激的cAMP积累增强。在海马体中，ADX增加了对异丙肾上腺素（ISOP）的cAMP反应，并降低了对组胺（HIST）的反应。在杏仁核和隔区中，ADX对ISOP的作用没有显著影响，但ADX确实降低了杏仁核对组胺的反应。地塞米松或皮质酮的给药逆转了ADX对海马体中cAMP积累的影响。地塞米松对VIP刺激的cAMP积累的作用在48小时内发生，并且在VIP剂量反应曲线的中间范围内最为明显。这些结果表明，糖皮质激素以一种特定的方式调节神经递质刺激的cAMP生成，这种方式对于所涉及的神经递质和受影响的脑区都是特异性的。
