Ming Hong, Ji Wei-Li, Li Shuai, Zhao Zhuo-Li, Zhang Ling-Yu, Meng Xiao-Lin, Zhou En-Min, Nie Guo-Xing, Li Wen-Jun
Synthetic Biology Remaking Engineering and Application Laboratory (SynBreal), College of Life Sciences and Technology, Xinxiang Medical University, Xinxiang 453003, PR China.
Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2953-2958. doi: 10.1099/ijsem.0.002057. Epub 2017 Aug 18.
A novel themophilic bacterium, designated YIM 79486T, was isolated from a sediment sample collected from Jinze hot spring in Tengchong county, Yunnan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequence analysis revealed that strain YIM 79486T should be assigned to the genus Laceyella and formed a monophyletic clade with the type strain Laceyella putida KCTC 3666T (98.7 % similarity). Strain YIM 79486T formed white aerial mycelium and brown substrate mycelium. Abundant endospores were produced on short sporophores. Cell-wall peptidoglycan contained meso-diaminopimelic acid. The predominant menaquinones were MK-9 and MK-8. The genomic DNA G+C content observed for strain YIM 79486T was 47.8 mol%. Based on low DNA-DNA hybridization data, chemotaxonomic characteristics and differential physiological properties, strain YIM 79486T is considered to represent a novel species within the genus Laceyella, for which the name Laceyella thermophila sp. nov. is proposed. The type strain is YIM 79486T (=CCTC AB 2015040T=NBRC 110772T).
从中国西南部云南省腾冲县金泽温泉采集的沉积物样本中分离出一株新型嗜热细菌，命名为YIM 79486T。基于16S rRNA基因序列分析的系统发育分析表明，菌株YIM 79486T应归属于Laceyella属，并与模式菌株恶臭Laceyella putida KCTC 3666T形成一个单系分支（相似性为98.7%）。菌株YIM 79486T形成白色气生菌丝体和棕色基内菌丝体。在短孢子梗上产生大量内生孢子。细胞壁肽聚糖含有内消旋二氨基庚二酸。主要的甲基萘醌是MK-9和MK-8。菌株YIM 79486T的基因组DNA G+C含量为47.8 mol%。基于低DNA-DNA杂交数据、化学分类特征和差异生理特性，菌株YIM 79486T被认为代表Laceyella属内的一个新物种，为此提出新物种名称嗜热Laceyella thermophila sp. nov.。模式菌株为YIM 79486T（=CCTC AB 2015040T=NBRC 110772T）。
