Li Dou-Nan, Huang Wei, Qiu Shu-Yi
Guizhou Provincial Key Laboratory of Fermentation Engineering and Biological Pharmacy, Guizhou university, Guiyang, 550025, PR China.
College of Life Sciences, Guizhou University, Guiyang, 550025, PR China.
Int J Syst Evol Microbiol. 2019 Sep;69(9):2709-2716. doi: 10.1099/ijsem.0.003528.
Moutai-flavour Daqu is an important starter to support growth of microorganisms in the fermented process of Moutai-flavour liquor. A novel thermophilic microorganism, designated strain FBKL4.011, was isolated from Moutai-flavour Daqu samples collected from Guotai distillery in Renhuai, Guizhou province, south-west China. The strain could grow at 45-65 °C (optimum 45 °C). Based on polyphasic analysis, strain FBKL4.011 was affiliated to the genus . It formed abundant pale-yellow aerial and substrate mycelium, bearing single endospores (7.0-10.0 µm diameter) on branched long sporophores (5.0 µm diameter). The cell-wall peptidoglycan contained -diaminopimelic acid; ribose, glucose and mannose were the primary whole-cell sugars. The major fatty acids were iso-C, iso-C, anteiso-C and iso-C. The predominant menaquinone were MK-8 and MK-9. The polar phospholipids contained diphosphatidyl glycerol, phosphatidyl ethanolamine, one unidentified phospholipid and one unidentified lipid. The G+C content of the genome was 43.1 mol%. According to the 16S rRNA gene sequence analysis, strain FBKL4.011 was closely related to JCM 9688 (95.3 % sequence similarity), and other members within the family (less than 93.0 % sequence similarity). The DNA-DNA hybridisation data showed low relatedness between strain FBKL4.011 and JCM 9688, KCTC 9790, YIM 10002, RHA1(36.7±1.1 %, 30.0±1.2 %, 21.3±2.1 % % and 37.6±0.9 %, respectively). Based on data from the polyphasic analysis, strain FBKL4.011 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FBKL4.011(=KCTC 43036=CICC 24504).
酱香型大曲是酱香型白酒发酵过程中微生物生长的重要发酵剂。从中国西南部贵州省仁怀市国泰酒厂采集的酱香型大曲样品中分离出一株新的嗜热微生物，命名为FBKL4.011菌株。该菌株能在45-65°C生长（最适温度45°C）。基于多相分析，FBKL4.011菌株属于 属。它形成丰富的浅黄色气生菌丝体和基内菌丝体，在分枝的长孢子梗（直径5.0μm）上产生单个内生孢子（直径7.0-10.0μm）。细胞壁肽聚糖含有 -二氨基庚二酸；核糖、葡萄糖和甘露糖是主要的全细胞糖。主要脂肪酸为异-C、异-C、anteiso-C和异-C。主要的甲基萘醌是MK-8和MK-9。极性磷脂含有双磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的磷脂和一种未鉴定的脂质。基因组的G+C含量为43.1mol%。根据16S rRNA基因序列分析，FBKL4.011菌株与JCM 9688密切相关（序列相似性为95.3%），与该科其他成员（序列相似性小于93.0%）。DNA-DNA杂交数据显示FBKL4.011菌株与JCM 9688、KCTC 9790、YIM 10002、RHA1之间的相关性较低（分别为36.7±1.1%、30.0±1.2%、21.3±2.1%和37.6±0.9%）。基于多相分析数据，FBKL4.011菌株被认为代表 属的一个新物种，为此提出新种名称 sp. nov.。模式菌株为FBKL4.011（=KCTC 43036=CICC 24504）。
