a Cardiovascular Research Institute , University of California San Francisco , San Francisco , CA , USA.
c Department of Laboratory of Medicine , University of California San Francisco, School of Medicine , San Francisco , CA , USA.
Cell Cycle. 2017 Oct 2;16(19):1835-1847. doi: 10.1080/15384101.2017.1361569. Epub 2017 Aug 18.
The emergence of haematopoietic stem and progenitor cells (HSPCs) from hemogenic endothelium results in the formation of sizeable HSPC clusters attached to the vascular wall. We evaluate the cell cycle and proliferation of HSPCs involved in cluster formation, as well as the molecular signatures from their initial appearance to the point when cluster cells are capable of adult engraftment (definitive HSCs). We uncover a non-clonal origin of HSPC clusters with differing cell cycle, migration, and cell signaling attributes. In addition, we find that the complement cascade is highly enriched in mature HSPC clusters, possibly delineating a new role for this pathway in engraftment.
造血干细胞和祖细胞（HSPCs）从造血内皮细胞中出现，导致附着在血管壁上的大量 HSPC 簇的形成。我们评估了参与簇形成的 HSPC 的细胞周期和增殖，以及从最初出现到簇细胞能够进行成人移植（确定性 HSCs）的分子特征。我们发现 HSPC 簇具有不同的细胞周期、迁移和细胞信号属性的非克隆起源。此外，我们发现补体级联在成熟的 HSPC 簇中高度富集，这可能为该途径在移植中的新作用提供了线索。
