Chemistry Department, Faculty of Science, Cairo University, 12613 Giza, Egypt.
Chemistry Department, Faculty of Science, Cairo University, 12613 Giza, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Jan 15;189:239-249. doi: 10.1016/j.saa.2017.08.001. Epub 2017 Aug 9.
A simple, fast and accurate spectrophotometric method had been developed to determine lead (II), chromium (III) and barium (II) ions in pure forms and in spiked water samples using thoron (THO) as a reagent forming colored complexes. It was found that the formed complexes absorbed maximally at 539, 540 and 538nm for Pb(II)-THO, Cr(III)-THO and Ba(II)-THO complexes, respectively. The optimum experimental conditions for these complexes had been studied carefully. Beer's law was obeyed in the range 1-35, 1-70, and 1-45μgmL for Pb (II), Cr(III) and Ba(II) ions with THO reagent, respectively. Different parameters such as linearity, selectivity, recovery, limits of quantification and detection, precision and accuracy were also evaluated in order to validate the proposed method. The results showed that, THO was effective in simultaneous determination of Pb(II), Cr(III) and Ba(III) ions in pure forms and in spiked water samples. Also, the results of the proposed method were compared with that obtained from atomic absorption spectrometry. The isolated solid complexes had been characterized using elemental analysis, X-ray powder diffraction (XRD), IR, mass spectrometry and TD-DFT calculations. Their biological activities were investigated against different types of bacteria and fungi organisms.
一种简单、快速、准确的分光光度法已经被开发出来，用于在纯形式和加标水样中测定铅（II）、铬（III）和钡（II）离子，使用钍（THO）作为形成有色络合物的试剂。研究发现，形成的络合物在 539、540 和 538nm 处对 Pb(II)-THO、Cr(III)-THO 和 Ba(II)-THO 络合物的吸收达到最大值。仔细研究了这些络合物的最佳实验条件。在 1-35、1-70 和 1-45μgmL 范围内，Pb(II)、Cr(III)和 Ba(II)离子与 THO 试剂的 Beer 定律都得到了遵守。为了验证所提出的方法，还评估了不同的参数，如线性、选择性、回收率、定量和检测限、精密度和准确性。结果表明，THO 能有效地同时测定纯形式和加标水样中的 Pb(II)、Cr(III)和 Ba(III)离子。此外，所提出的方法的结果与原子吸收光谱法的结果进行了比较。用元素分析、X 射线粉末衍射（XRD）、IR、质谱和 TD-DFT 计算对分离出的固体络合物进行了表征。研究了它们对不同类型的细菌和真菌生物的生物活性。
