High Pressure Processes Group, Department of Chemical Engineering and Environmental Technology, EII Sede Mergelina, University of Valladolid, 47011 Valladolid, Spain.
High Pressure Processes Group, Department of Chemical Engineering and Environmental Technology, EII Sede Mergelina, University of Valladolid, 47011 Valladolid, Spain.
Carbohydr Polym. 2017 Oct 15;174:1114-1120. doi: 10.1016/j.carbpol.2017.07.038. Epub 2017 Jul 17.
Barley and yeast β-glucans were selected, together with lecithin, to encapsulate resveratrol by emulsification-evaporation method to develop new and safer antifungal formulations. Different emulsification techniques were used: high-shear, high pressure and high pressure and temperature emulsification. Morphology, crystallinity, encapsulation efficiency and in vitro antifungal activity against Botrytis cinerea of the different formulations were evaluated. No significant differences between each emulsification procedure in particle size (below 90nm) and in encapsulation efficiency (70-100%) were observed; only barley β-glucan emulsions showed lower efficiency due to the formation of a gel that retained most of the active compound. A great influence of the emulsification method and the encapsulating material on the crystallinity of the particles was observed. The highest antifungal activity (up to 53% growth inhibition) was obtained by the formulations with yeast β-glucans, indicating an enhanced absorption of encapsulated resveratrol through the cell wall of the fungus at the presence of (1-3, 1-6)-β-glucans.
大麦和酵母β-葡聚糖与卵磷脂一起，通过乳化蒸发法被选择来包封白藜芦醇，以开发新的和更安全的抗真菌制剂。使用了不同的乳化技术：高剪切、高压和高压及温度乳化。对不同配方的形态、结晶度、包封效率和体外抗灰葡萄孢菌活性进行了评价。在粒径（低于 90nm）和包封效率（70-100%）方面，各乳化程序之间没有显著差异；只有大麦β-葡聚糖乳液的效率较低，因为形成了一种凝胶，保留了大部分活性化合物。乳化方法和包埋材料对颗粒结晶度有很大的影响。用酵母β-葡聚糖得到的制剂具有最高的抗真菌活性（高达 53%的生长抑制），这表明在（1-3,1-6）-β-葡聚糖存在下，包封的白藜芦醇通过真菌细胞壁的吸收得到增强。
