College of Food Science, Southwest University, Chongqing 400715, China; College of Food, XinYang Agriculture and Forestry University, XinYang 464000, China.
College of Food Science, Southwest University, Chongqing 400715, China.
Int J Biol Macromol. 2021 Jul 1;182:1192-1199. doi: 10.1016/j.ijbiomac.2021.05.043. Epub 2021 May 12.
In this study, four extraction methods, including ultrasonic extraction (UE), hot water extraction (HWE), microwave extraction (ME), and microwave-assisted ultrasonic extraction (MUE) were utilized to extract β-glucan from hull-less barley bran. Extraction yields and physicochemical properties of β-glucans extracted by different methods were investigated. The MUE displayed a highest extraction yield (2.16%) within shorter extraction time. Besides, β-glucans extracted by MUE (MUE-G) had higher number-average molecular weight (M (3.415 × 10), whereas β-glucans extracted by UE (UE-G) showed lower M (2.257 × 10) as compared to other methods. Accordingly, apparent viscosity of MUE-G was highest, while water solubility index of UE-G was highest (34.18-88.81%) at tested temperature ranges (25-95 °C). The MUE-G shower stronger foam stability and emulsifying properties and weaker foaming capability, while UE-G exhibited stronger foaming capability. The foaming capability and emulsifying properties of β-glucan products were better in neutral solutions (pH = 7) than in the acidic (pH = 4) and alkaline (pH = 9).
在这项研究中,采用了四种提取方法,包括超声提取(UE)、热水提取(HWE)、微波提取(ME)和微波辅助超声提取(MUE),从无壳大麦糠中提取β-葡聚糖。研究了不同方法提取的β-葡聚糖的提取率和理化性质。MUE 在较短的提取时间内显示出最高的提取率(2.16%)。此外,MUE 提取的β-葡聚糖(MUE-G)的数均分子量(M(3.415×10)较高,而 UE 提取的β-葡聚糖(UE-G)的 M 较低(2.257×10)与其他方法相比。因此,MUE-G 的表观粘度最高,而 UE-G 的水溶解度指数(25-95°C 测试温度范围内为 34.18-88.81%)最高。MUE-G 的泡沫稳定性和乳化性能较强,而 UE-G 的起泡能力较强。β-葡聚糖产品的起泡能力和乳化性能在中性溶液(pH=7)中优于酸性(pH=4)和碱性(pH=9)溶液。