Institute of Biomedical Engineering, Jinan University, Guangzhou 510632, PR China; Agricultural Product Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524001, PR China.
Institute of Biomedical Engineering, Jinan University, Guangzhou 510632, PR China.
Carbohydr Polym. 2017 Oct 15;174:432-442. doi: 10.1016/j.carbpol.2017.06.112. Epub 2017 Jun 29.
We prepared a novel hemostatic material using chitosan (CS), alginate, and Bletilla striata, which showed good biocompatibility and hemostatic properties. Alginate-calcium porous microspheres (Alg) were prepared by emulsification/cross-linking methods, and Bletilla striata polysaccharide (Bsp) was extracted from the tubers by water extraction and alcohol precipitation. To improve the water absorption and accelerate the blood coagulation, Alg and Bsp were mixed with chitosan solution to form CS/Alg and CS/Alg/Bsp composite sponges by lyophilization. Based on SEM images, the microspheres exhibited good spherical shape, possessed many pores on the surface, and were well dispersed in the CS/Alg5 and CS/Alg10 composite sponges. The CS/Alg10 composite sponges showed higher water absorption and porosity. In addition, the cytotoxicity assay and hemostatic property of the CS/Alg and CS/Alg/Bsp composite sponges were tested by CCK-8 assay, in vitro blood clotting, red blood cell (RBC) adhesion, and rabbit ear artery bleeding. These ternary composite sponges could be used as potential novel hemostatic materials in surgical treatment.
我们制备了一种新型的止血材料，该材料使用壳聚糖（CS）、海藻酸钠和白芨，具有良好的生物相容性和止血性能。海藻酸钠-钙多孔微球（Alg）通过乳化/交联方法制备，白芨多糖（Bsp）从块茎中用水提取和醇沉淀提取。为了提高吸水性并加速血液凝固，Alg 和 Bsp 与壳聚糖溶液混合，通过冷冻干燥形成 CS/Alg 和 CS/Alg/Bsp 复合海绵。基于 SEM 图像，微球呈现出良好的球形，表面具有许多孔，并且在 CS/Alg5 和 CS/Alg10 复合海绵中很好地分散。CS/Alg10 复合海绵具有更高的吸水性和孔隙率。此外，通过 CCK-8 测定、体外凝血、红细胞（RBC）黏附以及兔耳动脉出血试验测试了 CS/Alg 和 CS/Alg/Bsp 复合海绵的细胞毒性和止血性能。这些三元复合海绵可用作外科治疗中潜在的新型止血材料。
