Xie Jing, Zhang Li, Zeng Jin-Xiang, Li Min, Wang Juan, Xie Xiong-Xiong, Zhong Guo-Yue, Luo Guang-Ming, Yuan Jin-Bin, Liang Jian
Research Center of Traditional Chinese Medicine Resources and Minority Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
Department of Budget and Finance, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
Zhongguo Zhong Yao Za Zhi. 2017 Jun;42(11):2123-2130. doi: 10.19540/j.cnki.cjcmm.20170307.012.
The chemical constituents of Lagotis brevituba were rapidly determined and analyzed by using ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS/MS) method, providing material basis for the clinical application of L. brevituba. The separation was performed on UPLC YMC-Triart C₁₈ (2.1 mm×100 mm, 1.9 μm) column, with acetonitrile-water containing 0.2% formic acid as mobile phase for gradient elution. The flow rate was 0.4 mL•min-1 gradient elution and column temperature was 40 ℃, the injection volume was 2 μL. ESI ion source was used to ensure the data collected in a negative ion mode. The chemical components of L. brevituba were identified through retention time, exact relative molecular mass, cleavage fragments of MS/MS and reported data. The results showed that a total of 22 compounds were identified, including 11 flavones, 6 phenylethanoid glycosides, 1 iridoid glucosides, and 4 organic acid. The UPLC-Q-TOF-MS/MS method could fast identify the chemical components of L. brevituba, providing valuable information about L. brevituba for its clinical application.
采用超高效液相色谱串联四极杆飞行时间质谱（UPLC-Q-TOF-MS/MS）法对短管兔耳草的化学成分进行快速测定和分析，为短管兔耳草的临床应用提供物质基础。分离在UPLC YMC-Triart C₁₈（2.1 mm×100 mm，1.9 μm）柱上进行，以含0.2%甲酸的乙腈-水为流动相进行梯度洗脱。流速为0.4 mL•min⁻¹，梯度洗脱，柱温为40℃，进样量为2 μL。采用电喷雾离子源，确保数据在负离子模式下采集。通过保留时间、精确相对分子质量、MS/MS的裂解碎片和报道数据对短管兔耳草的化学成分进行鉴定。结果表明，共鉴定出22种化合物，包括11种黄酮类、6种苯乙醇苷类、1种环烯醚萜苷类和4种有机酸。UPLC-Q-TOF-MS/MS法可快速鉴定短管兔耳草的化学成分，为其临床应用提供有关短管兔耳草的有价值信息。
