Liu Ren-Yuan, Huang Fei-Ying, Guo Min, Lu Hai-Lin, Zhu Kai-Xin, Qin Wen-Hui, Qin Zhan-Min, Li Yong-Hua, Su Ben-Wei
Guangxi University of Chinese Medicine Nanning 530200, China.
Qinzhou Institute of Traditional Chinese Medicine Qinzhou 535000, China.
Zhongguo Zhong Yao Za Zhi. 2019 Jun;44(11):2283-2291. doi: 10.19540/j.cnki.cjcmm.20190415.202.
To build up an identification method on cardiac glycosides in Taxillus chinensis and its Nerium indicum host, and evaluate the influence on medicine quality from host to T. chinensis, ultra-performance liquid chromatography coupled with quadrupole-time-of-flight tandem mass-mass spectrometry(UPLC-Q-TOF-MS/MS)was applied. The samples of T. chinensis(harvested from N. indicum)and its N. indicum host were collected in field. The samples of T. chinensis(harvested from Morus alba)and its M. alba host was taken as control substance. All samples were extracted by ultrasonic extraction in 70% ethanol. Chromatographic separation was performed on an ACQUITY UPLC HSS T3 C_(18)(2.1 mm×100 mm,1.8 μm)column at 40 ℃. Gradient elution was applied, and the mobile phase was consisted of 0.1% formic acid water and acetonitrile. The 0.5 μL of sample solution was injected and the flow rate of the mobile phase was kept at 0.6 mL·min~(-1) in each run. It was done to identify cardiac glycosides and explore the chemical composition correlation in T. chinensis and its N. indicum host by analyzing positive and negative ion mode mass spectrometry data, elemental composition, cardiac glycoside reference substance and searching related literatures. A total of 29 cardiac glycosides were identified, 28 of it belonged to N. indicum host, 5 belonged to T. chinensis(harvested from N. indicum host), none of cardiac glycoside was identified in T. chinensis(harvested from M. alba host). The result could provide a reference in evaluating the influence in T. chinensis medicine quality from host. It was rapid, accurate, and comprehensive to identify cardiac glycosides in T. chinensis and its N. indicum host by UPLC-Q-TOF-MS/MS.
为建立桑寄生及其寄主夹竹桃中强心苷的鉴别方法，并评估寄主对桑寄生药材质量的影响，采用超高效液相色谱-四极杆-飞行时间串联质谱联用技术（UPLC-Q-TOF-MS/MS）。在田间采集了寄生于夹竹桃上的桑寄生及其夹竹桃寄主的样品，以寄生于桑树上的桑寄生及其桑树寄主的样品作为对照品。所有样品均用70%乙醇超声提取。色谱分离在ACQUITY UPLC HSS T3 C₁₈（2.1 mm×100 mm，1.8 μm）色谱柱上于40℃进行，采用梯度洗脱，流动相由0.1%甲酸水和乙腈组成。进样量为0.5 μL，每次运行时流动相流速保持在0.6 mL·min⁻¹。通过分析正、负离子模式质谱数据、元素组成、强心苷对照品并查阅相关文献，对强心苷进行鉴别并探究桑寄生及其夹竹桃寄主中的化学成分相关性。共鉴定出29种强心苷，其中28种属于夹竹桃寄主，5种属于寄生于夹竹桃上的桑寄生，寄生于桑树上的桑寄生中未鉴定出强心苷。该结果可为评估寄主对桑寄生药材质量的影响提供参考。采用UPLC-Q-TOF-MS/MS对桑寄生及其夹竹桃寄主中的强心苷进行鉴别，具有快速、准确、全面的特点。
