非酒精性脂肪性肝病患者的肠道菌群失调。

Gut microbiota dysbiosis in patients with non-alcoholic fatty liver disease.

机构信息

Department of Gastroenterology, Shanghai, China.

Research and Therapy Centre for Liver Disease, Zhengxing Hospital, Zhangzhou 363000, China.

出版信息

Hepatobiliary Pancreat Dis Int. 2017 Aug 15;16(4):375-381. doi: 10.1016/S1499-3872(17)60019-5.

DOI:10.1016/S1499-3872(17)60019-5

PMID:28823367

Abstract

BACKGROUND

Gut microbiota plays a significant role in the pathogenesis of non-alcoholic fatty liver disease (NAFLD). This study aimed to assess the contribution of gut microbiota dysbiosis to the pathogenesis of NAFLD.

METHODS

Forty-seven human feces samples (25 NAFLD patients and 22 healthy subjects) were collected and 16S rDNA amplicon sequencing was conducted on Hiseq 2000 platform. Discrepancy of species composition between controls and NAFLD group was defined by Metastats analysis under P value <0.01.

RESULTS

NAFLD patients harbored lower gut microbiota diversity than healthy subjects did. In comparison to the control group, the Proteobacteria (13.50%) and Fusobacteria (2.76%) phyla were more abundant in NAFLD patients. Additionally, the Lachnospiraceae (21.90%), Enterobacteriaceae (12.02%), Erysipelotrichaceae (3.83%), and Streptococcaceae (1.39%) families, as well as the Escherichia_Shigella (10.84%), Lachnospiraceae_Incertae_Sedis (7.79%), and Blautia (4.95%) genera were enriched in the NAFLD group. However, there was a lower abundance of Prevotella in the NAFLD group than that in the control group (5.83% vs 27.56%, P<0.01). The phylum Bacteroidetes (44.63%) also tended to be more abundant in healthy subjects, and the families Prevotellaceae (28.66%) and Ruminococcaceae (26.44%) followed the same trend. Compared to those without non-alcoholic steatohepatitis (NASH), patients with NASH had higher abundance of genus Blautia (5.82% vs 2.25%; P=0.01) and the corresponding Lachnospiraceae family (24.33% vs 14.21%; P<0.01). Patients with significant fibrosis had a higher abundance of genus Escherichia_Shigella (12.53% vs 1.97%; P<0.01) and the corresponding Enterobacteriaceae family (13.92% vs 2.07%; P<0.01) compared to those with F0/F1 fibrosis.

CONCLUSIONS

NAFLD patients and healthy subjects harbor varying gut microbiota. In contrast to the results of previous research on children, decreased levels of Prevotella might be detrimental for adults with NAFLD. The increased level of the genus Blautia, the family Lachnospiraceae, the genus Escherichia_Shigella, and the family Enterobacteriaceae may be a primary contributor to NAFLD progression.

摘要

背景

肠道微生物群在非酒精性脂肪性肝病（NAFLD）的发病机制中起着重要作用。本研究旨在评估肠道微生物群失调对 NAFLD 发病机制的贡献。

方法

收集了 47 个人类粪便样本（25 名 NAFLD 患者和 22 名健康受试者），并在 Hiseq 2000 平台上进行了 16S rDNA 扩增子测序。在 P 值 <0.01 的情况下，通过 Metastats 分析定义了对照组和 NAFLD 组之间物种组成的差异。

结果

NAFLD 患者的肠道微生物多样性低于健康受试者。与对照组相比，NAFLD 患者的厚壁菌门（13.50%）和梭杆菌门（2.76%）更为丰富。此外，lachnospiraceae 科（21.90%）、肠杆菌科（12.02%）、红螺菌科（3.83%）和链球菌科（1.39%）以及大肠杆菌-志贺氏菌属（10.84%）、lachnospiraceae_Incerta_Sedis 属（7.79%）和布劳特氏菌属（4.95%）在 NAFLD 组中富集。然而，NAFLD 组中普雷沃氏菌的丰度低于对照组（5.83%比 27.56%，P<0.01）。拟杆菌门（44.63%）在健康受试者中也倾向于更丰富，而 prevotellaceae 科（28.66%）和 rumino coccaceae 科（26.44%）也呈现出同样的趋势。与非酒精性脂肪性肝炎（NASH）患者相比，患有 NASH 的患者布劳特氏菌属（5.82%比 2.25%；P=0.01）和相应的lachnospiraceae 科（24.33%比 14.21%；P<0.01）的丰度更高。与 F0/F1 纤维化患者相比，具有显著纤维化的患者大肠杆菌-志贺氏菌属（12.53%比 1.97%；P<0.01）和相应的肠杆菌科（13.92%比 2.07%；P<0.01）的丰度更高。