State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, No. 2699 Qianjin Street, Changchun 130012, China.
Department of Foundation, Jilin Business and Technology College, No. 1666 Kalunhu Street, Changchun 130507, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Jan 15;189:291-299. doi: 10.1016/j.saa.2017.08.014. Epub 2017 Aug 9.
Two-dimensional correlation spectroscopy (2DCOS) has been widely used to Infrared, Raman, Near IR, Optical Activity (ROA), Vibrational Circular Dichroism (VCD) and Fluorescence spectroscopy. In addition, several new developments, such as 2D hetero-correlation analysis, moving-window two-dimensional (MW2D) correlation, model based correlation (βν and kν correlation analyses) have also well incorporated into protein research. They have been used to investigate secondary structure, denaturation, folding and unfolding changes of protein, and have contributed greatly to the field of protein science. This review provides an overview of the applications of 2DCOS in the field of protein science for the past 20 year, especially to memory our old friend, Dr. Boguslawa Czarnik-Matusewicz, for her great contribution in this research field. The powerful utility of 2DCOS combined with various analytical techniques in protein studies is summarized. The noteworthy developments and perspective of 2DCOS in this field are highlighted finally.
二维相关光谱学（2DCOS）已被广泛应用于红外、拉曼、近红外、旋光（ROA）、振动圆二色性（VCD）和荧光光谱学。此外，一些新的发展，如二维异相关分析、移动窗口二维（MW2D）相关、基于模型的相关（βν 和 kν 相关分析）也很好地应用于蛋白质研究中。它们被用于研究蛋白质的二级结构、变性、折叠和展开变化，并为蛋白质科学领域做出了巨大贡献。本综述概述了过去 20 年来 2DCOS 在蛋白质科学领域的应用，特别是为了纪念我们的老朋友 Boguslawa Czarnik-Matusewicz 博士，她在这一研究领域做出了巨大贡献。总结了 2DCOS 与各种分析技术在蛋白质研究中的强大应用。最后强调了 2DCOS 在该领域的显著发展和前景。
