二维相关光谱学在蛋白质研究中的进展。

Advancements of two dimensional correlation spectroscopy in protein researches.

机构信息

State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, No. 2699 Qianjin Street, Changchun 130012, China.

出版信息

Spectrochim Acta A Mol Biomol Spectrosc. 2018 May 15;197:185-193. doi: 10.1016/j.saa.2018.01.039. Epub 2018 Jan 31.

DOI:10.1016/j.saa.2018.01.039

PMID:29409703

Abstract

The developments of two-dimensional correlation spectroscopy (2DCOS) applications in protein studies are discussed, especially for the past two decades. The powerful utilities of 2DCOS combined with various analytical techniques in protein studies are summarized. The emphasis is on the vibration spectroscopic techniques including IR, NIR, Raman and optical activity (ROA), as well as vibration circular dichroism (VCD) and fluorescence spectroscopy. In addition, some new developments, such as hetero-spectral 2DCOS, moving-window correlation, and model based correlation, are also reviewed for their utility in the investigation of the secondary structure, denaturation, folding and unfolding changes of protein. Finally, the new possibility and challenges of 2DCOS in protein research are highlighted as well.

摘要

本文讨论了二维相关光谱（2DCOS）技术在蛋白质研究中的应用进展，尤其聚焦于过去二十年的发展。文中总结了 2DCOS 与多种分析技术相结合在蛋白质研究中的强大应用。重点介绍了包括红外、近红外、拉曼和旋光（ROA）以及振动圆二色性（VCD）和荧光光谱在内的振动光谱技术。此外，还综述了一些新的发展，如异谱 2DCOS、移动窗口相关和基于模型的相关，以评估它们在研究蛋白质的二级结构、变性、折叠和展开变化方面的应用。最后，本文还强调了 2DCOS 在蛋白质研究中的新可能性和挑战。