Key Laboratory of Interfacial Reaction&Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Key Laboratory of Interfacial Reaction&Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Biosens Bioelectron. 2018 Jan 15;99:493-499. doi: 10.1016/j.bios.2017.08.034. Epub 2017 Aug 15.
In this work, a label-free photoelectrochemical (PEC) immunosensor for the detection of carcino embryonic antigen (CEA) was developed based on CdS nanowires (NWs) sensitized WO@BiOI heterostructure. The construction procedure of ITO/WO@BiOI@CdS electrode was done by dipping the WO@BiOI modified indium-tin oxide (ITO) electrode interchangeably into [Cd(NH)] and S solution for several times. Then the ITO/WO@BiOI@CdS electrode was used as a matrix for the subsequent immobilization of CEA antibody (Ab). The obtained label-free PEC immunosensor showed an excellent PEC performance toward CEA detection. Under optimal conditions, the PEC immunosensor have a sensitive response to CEA in a liner range of 0.01ng/mL to 50ng/mL with a detection limit of 3.2pg/mL. The proposed PEC immunosensor exhibited good stability, high sensitivity as well as reproducibility and storage stability. Moreover, the obtained PEC immunosensor also showed a satisfied result in human serum sample analysis.
在这项工作中，基于 CdS 纳米线（NWs）敏化 WO@BiOI 异质结，开发了一种用于检测癌胚抗原（CEA）的无标记光电流（PEC）免疫传感器。通过将 WO@BiOI 修饰的氧化铟锡（ITO）电极多次交替浸入[Cd(NH)]和 S 溶液中，完成了 ITO/WO@BiOI@CdS 电极的构建过程。然后，将 ITO/WO@BiOI@CdS 电极用作随后固定 CEA 抗体（Ab）的基质。所获得的无标记 PEC 免疫传感器对 CEA 检测表现出优异的 PEC 性能。在最佳条件下，PEC 免疫传感器对 0.01ng/mL 至 50ng/mL 范围内的 CEA 具有灵敏的响应，检测限为 3.2pg/mL。所提出的 PEC 免疫传感器表现出良好的稳定性、高灵敏度以及重现性和储存稳定性。此外，所获得的 PEC 免疫传感器在人血清样品分析中也显示出令人满意的结果。
