Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Biosens Bioelectron. 2019 Feb 1;126:23-29. doi: 10.1016/j.bios.2018.10.045. Epub 2018 Oct 24.
In this study, we propose a SnO/CdCO/CdS-based label-free photoelectrochemical (PEC) immunosensor for the sensitive quantification of amyloid β-protein (Aβ) which plays a critical role in the early diagnosis of Alzheimer's disease. Under specific pH conditions, SnO/CdCO/CdS was first designed and successfully synthesized with simple one-pot method. The synthesis of SnO/CdCO/CdS circumvents the trouble of traditional composite materials to be gradually compounded, saving a lot of time and energy. The synthesis mechanism of SnO/CdCO/CdS nanocomposite was also deeply explored. Moreover, thanks to the matched energy levels of its three components, SnO/CdCO/CdS nanocomposite exhibited enhanced photocurrent intensity, which is dozens of times bigger than any of its components, thus making it an excellent photoactive matrix for PEC immunosensor. Under optimal conditions, the as-constructed PEC immunosensor manifested a broad linear range (0.1 pg mL to 100 ng mL) with a low detection limit (LOD, 0.05 pg mL, S/N = 3). High selectivity, coupled with good stability, allowed the developed PEC immunosensor to be applied in the clinical detection of Aβ or other biomarkers in human serum.
在这项研究中，我们提出了一种基于 SnO/CdCO/CdS 的无标记光电流免疫传感器，用于灵敏地定量检测淀粉样 β 蛋白（Aβ），该蛋白在阿尔茨海默病的早期诊断中起着关键作用。在特定的 pH 条件下，我们首先采用简单的一锅法设计并成功合成了 SnO/CdCO/CdS。SnO/CdCO/CdS 的合成避免了传统复合材料逐渐复合的麻烦，节省了大量的时间和精力。我们还深入探索了 SnO/CdCO/CdS 纳米复合材料的合成机制。此外，由于其三个组成部分的能级匹配，SnO/CdCO/CdS 纳米复合材料表现出增强的光电流强度，是其任何组成部分的数十倍，因此使其成为用于 PEC 免疫传感器的优异光活性基质。在最佳条件下，所构建的 PEC 免疫传感器表现出宽线性范围（0.1 pg/mL 至 100 ng/mL）和低检测限（LOD，0.05 pg/mL，S/N=3）。高选择性，加上良好的稳定性，使开发的 PEC 免疫传感器能够应用于人类血清中 Aβ或其他生物标志物的临床检测。
