去甲肾上腺素对体外培养的大鼠下丘脑免疫反应性促肾上腺皮质激素释放因子释放的抑制作用。

Inhibitory effect of norepinephrine on immunoreactive corticotropin-releasing factor release from the rat hypothalamus in vitro.

作者信息

Suda T, Yajima F, Tomori N, Sumitomo T, Nakagami Y, Ushiyama T, Demura H, Shizume K

出版信息

Life Sci. 1987 Apr 27;40(17):1645-9. doi: 10.1016/0024-3205(87)90012-9.

Abstract

Effects of catecholamines on immunoreactive corticotropin-releasing factor (I-CRF) release from the rat hypothalamus were examined using a rat hypothalamic perifusion system and a rat CRF RIA in vitro. Norepinephrine had a potent inhibitory effect on I-CRF release in a dose-dependent manner at 0.1 nM-1 microM concentrations, but dopamine did not. This inhibitory effect of norepinephrine was completely blocked by propranolol, but only partially blocked by phentolamine. Isoproterenol also had a potent inhibitory effect at 0.01-100 nM concentrations, and a high dose of phenylephrine (10 nM) inhibited I-CRF release. Clonidine did not influence I-CRF release. These results suggest that norepinephrine inhibits I-CRF release mainly through the beta-adrenergic receptor and partially through the alpha 1-receptor.

摘要

利用大鼠下丘脑灌流系统和大鼠促肾上腺皮质激素释放因子放射免疫分析方法，在体外研究了儿茶酚胺对大鼠下丘脑免疫反应性促肾上腺皮质激素释放因子（I-CRF）释放的影响。去甲肾上腺素在0.1 nM - 1 μM浓度范围内对I-CRF释放具有剂量依赖性的强烈抑制作用，而多巴胺则无此作用。去甲肾上腺素的这种抑制作用可被普萘洛尔完全阻断，但仅被酚妥拉明部分阻断。异丙肾上腺素在0.01 - 100 nM浓度范围内也具有强烈抑制作用，高剂量的去氧肾上腺素（10 nM）可抑制I-CRF释放。可乐定不影响I-CRF释放。这些结果表明，去甲肾上腺素主要通过β-肾上腺素能受体抑制I-CRF释放，部分通过α1-受体发挥作用。