Haraguchi Kazutomo
National Food Research Institute, 2-1-12 Kannondai, Tsukuba-shi, Ibaraki 305-8642, Japan.
Materials (Basel). 2011 Sep 7;4(9):1543-1547. doi: 10.3390/ma4091543.
Inulin is a polysaccharide contained in chicory, dahlia, and other plants. An oligosaccharide DFA III is produced from inulin using a microbial enzyme, inulin fructotransferase (DFA III producing) [EC 2.4.1.93]. The oligosaccharide DFAIII has a unique functionality that accelerates the assimilation of minerals (Ca, Fe, and so on) from intestines. Therefore, it has a potential for the improvement of osteoporosis and iron deficiency anemia. The production of DFA III was industrialized in 2004 in Japan. Another oligosaccharide DFA I is produced from inulin by another enzyme, inulin fructotransferase (DFA I producing) [EC 2.4.1.200]. The oligosaccharide DFA I has half the sweetness of sucrose. The genes of the two enzymes were cloned and the nucleotide sequences were determined. The deduced amino acid sequences of two enzyme genes had a homology of 49.8%.
菊粉是一种存在于菊苣、大丽花和其他植物中的多糖。使用微生物酶菊粉果糖转移酶（产生DFA III）[EC 2.4.1.93]可从菊粉中生产出低聚糖DFA III。低聚糖DFAIII具有独特的功能，能加速矿物质（钙、铁等）从肠道的吸收。因此，它在改善骨质疏松症和缺铁性贫血方面具有潜力。2004年，DFA III的生产在日本实现了工业化。另一种低聚糖DFA I是由另一种酶菊粉果糖转移酶（产生DFA I）[EC 2.4.1.200]从菊粉中生产出来的。低聚糖DFA I的甜度是蔗糖的一半。这两种酶的基因被克隆并测定了核苷酸序列。两种酶基因推导的氨基酸序列具有49.8%的同源性。
