定位与人类X染色体Xq27.3处脆性位点附近的DNA标记。
Mapping of DNA markers close to the fragile site on the human X chromosome at Xq27.3.
作者信息
Patterson M, Kenwrick S, Thibodeau S, Faulk K, Mattei M G, Mattei J F, Davies K E
出版信息
Nucleic Acids Res. 1987 Mar 25;15(6):2639-51. doi: 10.1093/nar/15.6.2639.
We report the identification of a new RFLP detected by the DNA probe MN12, which is linked to both the fragile site on the X chromosome at Xq27.3 and the highly polymorphic locus detected by St14 (DXS52). In situ mapping confirms the localisation of MN12 distal to the fragile site. A detailed physical analysis of this region of the X chromosome using pulsed-field gel electrophoresis has shown that MN12, St14 and DX13 (DXS15) are physically linked within a region of 470kb. A long range restriction map around the MN12 locus reveals at least two candidate HTF islands, suggesting the existence of expressed sequences in this region.
我们报告了通过DNA探针MN12检测到的一种新的限制性片段长度多态性（RFLP），它与X染色体上位于Xq27.3的脆性位点以及由St14（DXS52）检测到的高度多态性位点均相关联。原位定位证实了MN12位于脆性位点的远端。使用脉冲场凝胶电泳对X染色体的这一区域进行的详细物理分析表明，MN12、St14和DX13（DXS15）在470kb的区域内物理相连。围绕MN12基因座的长程限制性图谱揭示了至少两个候选的富含热稳定蛋白的岛，这表明该区域存在表达序列。
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