Almasry Shaima M, Elfayomy Amr K, El-Sherbiny Mohamed H
1 Department of Anatomy, Almansoura University, Egypt.
2 Department of Anatomy, Almadinah Almunawarah, Taibah University, Saudi Arabia.
Reprod Sci. 2018 May;25(5):773-781. doi: 10.1177/1933719117725824. Epub 2017 Aug 22.
In this study, we describe a novel insight about the use of bone marrow-derived mesenchymal stem cells (BM-MSCs) for fallopian tube (FT) regeneration. Seventy rats' tubes were involved in this study and divided into 4 groups: control (15), ethanol injured (20), mesenchymal stem cell (MSC)-recipient without injury (15), and MSC recipient after injury (20). The BM-MSCs were isolated from male rats, and their incorporation into the tissues was confirmed by the detection of Sry gene in MSC-recipient rats using RT-PCR. Histological and immunohistological sections of the 4 groups were comparably evaluated. We found that direct injection of ethanol into FT caused structural impairment, which was restored largely after receiving MSCs. We have revealed for the first time that prominin 1 (Prom1, stem cell marker) was expressed in the fimbriated distal tubal end. The MSC transplantation caused (1) significant increase in the tissue level and immunoexpresstion of Prom1 ( P < .001 and P = .017, respectively) and vascular endothelial growth factor (VEGF; vasculogenic marker; P < .001 and P = .004, respectively), (2) significant increase in the immunoexpresstion of proliferating cell nuclear antigen (PCNA; proliferation marker; P < .001), and (3) significant decrease in the immunoexpresstion of caspase 3 (CASP-3; apoptotic marker; P < .001) compared to the injured tissues. In conclusion, MSCs could exhibit its restorative effect on FT through their ability to (1) activate the resident stem cells in the distal tubal end, (2) mediate the expression of VEGF and PCNA, and (3) influence tissue apoptosis. This study laid the foundation for assessing the contribution of stem cells in the distal tubal end in direct repair of the tube when required to assist reproduction.
在本研究中，我们描述了关于使用骨髓间充质干细胞（BM-MSCs）进行输卵管（FT）再生的一项新见解。本研究纳入了70只大鼠的输卵管，并将其分为4组：对照组（15只）、乙醇损伤组（20只）、未损伤的间充质干细胞（MSC）受体组（15只）和损伤后的MSC受体组（20只）。从雄性大鼠中分离出BM-MSCs，并通过逆转录聚合酶链反应（RT-PCR）检测MSC受体大鼠中的Sry基因，证实其整合到组织中。对4组的组织学和免疫组织学切片进行了比较评估。我们发现，向FT直接注射乙醇会导致结构损伤，而在接受MSC后损伤在很大程度上得到恢复。我们首次发现，prominin 1（Prom1，干细胞标志物）在输卵管远端有纤毛的末端表达。与损伤组织相比，MSC移植导致：（1）Prom1的组织水平和免疫表达显著增加（分别为P <.001和P =.017）以及血管内皮生长因子（VEGF；血管生成标志物；分别为P <.001和P =.004），（2）增殖细胞核抗原（PCNA；增殖标志物）的免疫表达显著增加（P <.001），以及（3）半胱天冬酶3（CASP-3；凋亡标志物）的免疫表达显著降低（P <.001）。总之，MSCs可以通过以下能力对FT发挥其修复作用：（1）激活输卵管远端的驻留干细胞，（2）介导VEGF和PCNA的表达，以及（3）影响组织凋亡。本研究为评估干细胞在输卵管远端对辅助生殖所需的输卵管直接修复中的作用奠定了基础。
