Glorioso N, Troffa C, Laragh J H, Atlas S A, Marion D, Sealey J E
Am J Physiol. 1987 Apr;252(4 Pt 1):E509-18. doi: 10.1152/ajpendo.1987.252.4.E509.
Inactive renin, prorenin, is found in high concentrations in human plasma. We report herein the characteristics of trypsin-activated inactive renin from cat kidney and plasma. Cat and human plasma inactive renin were activated by similar concentrations of trypsin. As in humans, there was more inactive than active renin in cat plasma; also, inactive renin was low but detectable after nephrectomy. Trypsin-activated renal inactive renin, purified on Cibacron blue agarose and pepstatin-amino-hexyl-Sepharose chromatography, was inhibited by pepstatin and by a renin inhibitor similarly to cat and human active renins. The pH optimum of cat renin was biphasic: the higher peak of active renin was at pH 5.7, whereas that of activated inactive renin was at pH 7.5. As in humans, active and inactive plasma renin increased during sodium depletion and inactive renin increased during beta-adrenergic blockade, while active renin decreased. These results demonstrate that cat inactive renin is similar to human prorenin. Therefore, the cat may be a useful model for the study of prorenin.
无活性肾素即肾素原，在人血浆中含量很高。我们在此报告来自猫肾脏和血浆的经胰蛋白酶激活的无活性肾素的特性。猫和人血浆中的无活性肾素被相似浓度的胰蛋白酶激活。与人类一样，猫血浆中无活性肾素比活性肾素更多；此外，肾切除术后无活性肾素含量低但仍可检测到。经胰蛋白酶激活的肾脏无活性肾素，通过固定化蓝色琼脂糖和胃蛋白酶抑制剂 - 氨基己基 - 琼脂糖凝胶柱色谱法纯化后，与猫和人的活性肾素一样，受到胃蛋白酶抑制剂和肾素抑制剂的抑制。猫肾素的最适pH呈双相性：活性肾素的较高峰值在pH 5.7，而经激活的无活性肾素的较高峰值在pH 7.5。与人类一样，钠缺乏时血浆中活性和无活性肾素均增加，β - 肾上腺素能阻滞剂作用期间无活性肾素增加，而活性肾素减少。这些结果表明猫的无活性肾素与人类肾素原相似。因此，猫可能是研究肾素原的有用模型。
