Usharani Boopathy, Vasudevan Namasivayam
Centre for Environmental Studies, CEG Campus, Anna University, Chennai, Tamil Nadu, India.
Pharmacognosy Res. 2017 Jul-Sep;9(3):294-300. doi: 10.4103/pr.pr_107_16.
Secondary metabolites play a vital role in the treatment of various ailments as well as in phytoremediation. The link between secondary metabolites and phytoremediation needs exploration. Hitherto, no information is available regarding the phytochemical components that exist in the root exudates of . This study was designed to determine the phytocomponents in the root exudates of under heavy metal stress. was grown by a novel technique in partial hydroponic conditions and imperiled to a mixture of heavy metals (Cd, Cu, Cr, Ni, Zn, Pb, and Fe) at different concentrations. The root exudates were collected, freeze-dried, redissolved and reconstituted in hexane and analyzed in gas chromatography-mass spectrometry using JEOL GCMATE II in SAIF IIT-Madras. The analysis revealed that the profile of phytochemicals in root exudates is diverse with biological properties. Few phytochemicals found in the root exudates are not cited earlier in any literature. The composition and percentage of phytochemicals could not be correlated to heavy metal concentration. Phytochemical composition decreased with an increase in heavy metal concentration. Control plant released more phytochemicals than the plants under heavy metal stress. From the results, it is evident that root exudates of contain various bioactive components. Further research can be extended to evaluate the pharmaceutical importance of the species and explore its role in phytoremediation of heavy metals.
The control and test plants are grown under partial hydroponic condition. Test plants are subjected to heavy metal stress, root exudates were collected from control and test plants, freeze dried, constituted in hexane and subjected to GC-MS analysis. : GC-MS: Gas chromatography-Mass spectrometry.
次生代谢产物在各种疾病的治疗以及植物修复中发挥着至关重要的作用。次生代谢产物与植物修复之间的联系有待探索。迄今为止，关于[植物名称]根系分泌物中存在的植物化学成分尚无相关信息。本研究旨在确定[植物名称]在重金属胁迫下根系分泌物中的植物成分。[植物名称]通过一种新技术在部分水培条件下生长，并暴露于不同浓度的重金属（镉、铜、铬、镍、锌、铅和铁）混合物中。收集根系分泌物，冷冻干燥，重新溶解并在己烷中重构，然后在位于印度理工学院马德拉斯分校的SAIF使用JEOL GCMATE II进行气相色谱 - 质谱分析。分析表明，根系分泌物中的植物化学物质谱具有多种生物学特性。在根系分泌物中发现的一些植物化学物质在任何文献中都未曾有过记载。植物化学物质的组成和百分比与重金属浓度无关。随着重金属浓度的增加，植物化学成分减少。对照植物比遭受重金属胁迫的植物释放出更多的植物化学物质。从结果来看，显然[植物名称]的根系分泌物含有各种生物活性成分。可以进一步开展研究以评估该物种的药用价值，并探索其在重金属植物修复中的作用。
对照植物和试验植物在部分水培条件下生长。试验植物遭受重金属胁迫，从对照植物和试验植物中收集根系分泌物，冷冻干燥，在己烷中重构并进行气相色谱 - 质谱分析。：GC - MS：气相色谱 - 质谱联用。
