Irimia Ramona-Elena, Montesinos Daniel, Eren Özkan, Lortie Christopher J, French Kristine, Cavieres Lohengrin A, Sotes Gastón J, Hierro José L, Jorge Andreia, Loureiro João
Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, Coimbra, Portugal.
National Institute of Research and Development for Biological Sciences, Stejarul Research Centre for Biological Sciences, Piatra Neamt, Romania.
PeerJ. 2017 Aug 14;5:e3531. doi: 10.7717/peerj.3531. eCollection 2017.
L. (yellow starthistle, Asteraceae) is a Eurasian native plant introduced as an exotic into North and South America, and Australia, where it is regarded as a noxious invasive. Changes in ploidy level have been found to be responsible for numerous plant biological invasions, as they are involved in trait shifts critical to invasive success, like increased growth rate and biomass, longer life-span, or polycarpy. . had been reported to be diploid (2 = 2 = 16 chromosomes), however, actual data are scarce and sometimes contradictory. We determined for the first time the absolute nuclear DNA content by flow cytometry and estimated ploidy level in 52 natural populations of . across its native and non-native ranges, around the world. All the populations screened were found to be homogeneously diploid (average 2C value of 1.72 pg, SD = ±0.06 pg), with no significant variation in DNA content between invasive and non-invasive genotypes. We did not find any meaningful difference among the extensive number of native and non-native . populations sampled around the globe, indicating that the species invasive success is not due to changes in genome size or ploidy level.
L.（黄矢车菊，菊科）是一种原产于欧亚大陆的植物，作为外来物种被引入到北美洲、南美洲和澳大利亚，在这些地方它被视为有害的入侵物种。人们发现倍性水平的变化是许多植物生物入侵的原因，因为它们参与了对入侵成功至关重要的性状转变，如生长速率和生物量增加、寿命延长或多次结果。据报道， 为二倍体（2n = 2x = 16条染色体），然而，实际数据稀少且有时相互矛盾。我们首次通过流式细胞术测定了 52 个世界各地其原生和非原生分布范围内的自然种群的绝对核 DNA 含量并估计了倍性水平。所有被筛选的种群均被发现为均匀二倍体（平均 2C 值为 1.72 pg，标准差 = ±0.06 pg），入侵和非入侵基因型之间的 DNA 含量没有显著差异。我们在全球范围内采样的大量原生和非原生 种群中未发现任何有意义的差异，这表明该物种的入侵成功并非由于基因组大小或倍性水平的变化。
