使用多重报告基因追踪细胞外囊泡递送和RNA翻译

Tracking Extracellular Vesicles Delivery and RNA Translation Using Multiplexed Reporters.

作者信息

Wu Anthony Yan-Tang, Lai Charles Pin-Kuang

机构信息

Institute of Biomedical Engineering, National Tsing Hua University, No. 101, Section 2, Kuang-Fu Road, Hsinchu, 30013, Taiwan.

Institute of Atomic and Molecular Sciences, Academia Sinica, No. 1, Roosevelt Rd., Sec. 4, Taipei, 10617, Taiwan.

出版信息

Methods Mol Biol. 2017;1660:255-265. doi: 10.1007/978-1-4939-7253-1_21.

DOI:10.1007/978-1-4939-7253-1_21

PMID:28828663

Abstract

Elucidating extracellular vesicle (EV; e.g., exosomes, microvesicles) delivery and translation of its RNA cargo with an accurate spatiotemporal resolution is critical in helping understand EV's role under normal and pathological conditions. We here describe a multiplexed fluorescent and bioluminescent reporter strategy to simultaneously monitor and quantify EV delivery, as well as EV-RNA translation in EV-recipient cells.

摘要

以精确的时空分辨率阐明细胞外囊泡（EV；例如，外泌体、微囊泡）的递送及其RNA货物的翻译，对于帮助理解EV在正常和病理条件下的作用至关重要。我们在此描述了一种多重荧光和生物发光报告策略，用于同时监测和量化EV的递送以及EV受体细胞中EV-RNA的翻译。