Kenigsberg Shlomit, Wyse Brandon A, Librach Clifford L, da Silveira Juliano C
CreATe Fertility Centre, Toronto, ON, Canada.
Department of Obstetrics and Gynecology, University of Toronto, Toronto, ON, Canada.
Methods Mol Biol. 2017;1660:321-341. doi: 10.1007/978-1-4939-7253-1_26.
The ovarian follicular fluid (FF) is a complex fluid that constitutes the microenvironment of developing follicles and contains factors secreted by the surrounding cells and blood plasma compounds that cross the "blood-follicle barrier." Upon oocyte retrieval (in human, bovine, and equine) the follicular fluid is normally discarded and represents a repertoire of cellular messages exchanged during follicle development, thus providing a suitable sample for performing oocyte quality diagnostics. Several studies report on the presence of extracellular vesicles (EVs) in FF from human, bovine and equine. Here, we describe the process of FF collection from human and bovine and the enrichment and isolation of EVs that we termed folliculosomes (FFEs), using available commercial kits as well as the traditional ultracentrifugation methods.
卵巢卵泡液（FF）是一种复杂的液体，它构成了发育中卵泡的微环境，包含周围细胞分泌的因子以及穿过“血-卵泡屏障”的血浆成分。在取卵时（在人类、牛和马中），卵泡液通常会被丢弃，它代表了卵泡发育过程中交换的一系列细胞信息，因此为进行卵母细胞质量诊断提供了一个合适的样本。几项研究报道了人类、牛和马的卵泡液中存在细胞外囊泡（EVs）。在这里，我们描述了从人类和牛身上收集卵泡液的过程，以及使用现有的商业试剂盒和传统的超速离心方法对我们称为卵泡小体（FFEs）的细胞外囊泡进行富集和分离的过程。
