Da Silveira Juliano, Andrade Gabriella M, Perecin Felipe, Meireles Flávio Vieira, Winger Quinton A, Bouma Gerrit J
Faculty of Animal Sciences and Food Engineering, Department of Veterinary Medicine, University of Sao Paulo, Pirassununga, SP, Brazil.
College of Veterinary and Biomedical Sciences, Department of Biomedical Sciences, Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, CO, USA.
Methods Mol Biol. 2018;1733:53-63. doi: 10.1007/978-1-4939-7601-0_4.
Mammalian ovarian follicular growth is characterized by development of a large fluid filled antrum that separates mural granulosa cells and cumulus cells. Extensive communication between the different cell types is necessary for maturation of a developmentally competent oocyte. Here, we describe an approach for the isolation of cell-secreted exosomes from ovarian follicular fluid, identification of small RNAs (i.e., microRNAs) in exosomes, labeling of exosomes, and examining cell uptake of exosomes by follicular cells.
哺乳动物卵巢卵泡生长的特征是形成一个充满液体的大腔，该腔将壁层颗粒细胞和卵丘细胞分隔开来。不同细胞类型之间的广泛通讯对于发育能力成熟的卵母细胞的成熟是必要的。在这里，我们描述了一种从卵巢卵泡液中分离细胞分泌的外泌体、鉴定外泌体中的小RNA（即微小RNA）、对外泌体进行标记以及检测卵泡细胞对外泌体的细胞摄取的方法。
