疟疾中细胞外囊泡的产生与特性分析

Production and Characterization of Extracellular Vesicles in Malaria.

作者信息

Mbagwu Smart, Walch Michael, Filgueira Luis, Mantel Pierre-Yves

机构信息

Department of Medicine, Unit of Anatomy, University of Fribourg, Fribourg, Switzerland.

出版信息

Methods Mol Biol. 2017;1660:377-388. doi: 10.1007/978-1-4939-7253-1_31.

DOI:10.1007/978-1-4939-7253-1_31

PMID:28828673

Abstract

Growing attention is drawn toward the role of extracellular vesicles (EVs) in infectious diseases. EVs, which are small vesicles released by cells, are involved in cellular communication, immune regulation, and pathogenesis. EVs act as messenger carrying functional cargoes, including RNA, DNA, lipids and proteins from a donor cell to regulate the function of a recipient cell. In malaria, EVs play a key role in regulating the progression from the blood to the transmission stage by promoting the switch between asexual and sexual stages that are taken up by mosquitoes. In addition to their role in parasite communication, EVs modulate the immune system and regulate endothelial cell function.In this chapter, we describe protocols to isolate, purify and characterize EVs derived from Plasmodium falciparum infected red blood cell culture.

摘要

细胞外囊泡（EVs）在传染病中的作用日益受到关注。EVs是细胞释放的小囊泡，参与细胞通讯、免疫调节和发病机制。EVs作为携带功能性货物的信使，包括来自供体细胞的RNA、DNA、脂质和蛋白质，以调节受体细胞的功能。在疟疾中，EVs通过促进被蚊子摄取的无性和有性阶段之间的转换，在调节从血液阶段到传播阶段的进程中发挥关键作用。除了在寄生虫通讯中的作用外，EVs还调节免疫系统并调节内皮细胞功能。在本章中，我们描述了从恶性疟原虫感染的红细胞培养物中分离、纯化和表征EVs的方案。