Dental Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran. Iran.
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran. Iran.
Curr Stem Cell Res Ther. 2017;12(7):564-599. doi: 10.2174/1574888X12666170822100105.
Traditional attempts to grow bone grafts in vitro have been based on culturing cell-scaffold constructs under static culture conditions. However, limitations associated with this approach have led to the development of various types of technologies and equipments. One of these is a bioreactor acting as an intermediate between static (in vitro) and dynamic (in vivo) conditions, which can mimic physiological and mechanical body conditions.
The aim of this study was to systematically review the available literature on application of different types of bioreactors in bone tissue engineering.
A thorough search in PubMed and Google Scholar databases from January 2011 to December 2016 was performed. All in vitro and in vivo studies about bioreactor applications in bone tissue engineering were included and categorized according to bioreactor types.
A comprehensive systematic review of all the studies from the past five years yielded several findings: (1) combined bioreactors seem effective in bone tissue engineering; (2) 1- 2 ml/min is an appropriate flow rate range; (3) a cylinder is an appropriative scaffold shape; and (4) incubation of the scaffold with cells prior to transfer to the bioreactor followed by administration of osteogenic medium in the bioreactor seems an efficient approach to help cells properly attach and differentiate.
传统的体外培养骨移植物的尝试是基于在静态培养条件下培养细胞-支架构建体。然而，这种方法的局限性导致了各种类型的技术和设备的发展。其中之一是生物反应器，它作为静态（体外）和动态（体内）条件之间的中间物，可以模拟生理和机械的身体条件。
本研究的目的是系统地回顾可用于骨组织工程的不同类型生物反应器的应用文献。
对 2011 年 1 月至 2016 年 12 月在 PubMed 和 Google Scholar 数据库中进行了全面搜索。纳入了所有关于生物反应器在骨组织工程中的应用的体外和体内研究，并根据生物反应器类型进行分类。
对过去五年的所有研究进行全面系统的回顾得出了以下发现：（1）组合生物反应器似乎在骨组织工程中有效；（2）1-2ml/min 是合适的流速范围；（3）圆柱是合适的支架形状；（4）在将支架转移到生物反应器之前与细胞孵育，然后在生物反应器中给予成骨介质，这似乎是一种有效的方法，可以帮助细胞正确附着和分化。
