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祖细胞的共培养增强了成骨基因表达和血管生成潜力。

Co-cultivation of progenitor cells enhanced osteogenic gene expression and angiogenesis potential .

作者信息

Jia Yongsheng, Zhang Cuicui, Zheng Xiangqian, Gao Ming

机构信息

Thyroid and Neck Department, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, China.

Key Laboratory of Breast Cancer Prevention and Therapy, Tianjin Medical University, Ministry of Education, Tianjin, China.

出版信息

J Int Med Res. 2021 Apr;49(4):3000605211004024. doi: 10.1177/03000605211004024.

DOI:10.1177/03000605211004024
PMID:33840248
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8044578/
Abstract

OBJECTIVES

The efficiencies of osteogenesis and angiogenesis present challenges that need to be overcome before bone tissue engineering can be widely applied to clinical uses. We aimed to optimize an culture system to enhance osteogenesis and angiogenesis. We investigated if hematopoietic stem cells (HSCs) promoted osteogenesis when co-cultured with mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs).

METHODS

MSC/HSC, MSC/EPC/HSC, and MSC/EPC co-cultures were incubated for 21 days. Alkaline phosphatase (ALP) activity and calcium content were analyzed to assess mineralization. Expression levels of genes encoding osteogenesis-related proteins (ALP (), collagen type IA (), osteocalcin (), and osteopontin ()) were also evaluated by measuring mRNA levels at day 28. Angiogenesis was evaluated by tube-formation assay.

RESULTS

, , , and genes were upregulated in MSC/HSC and MSC/EPC/HSC co-cultures compared with the MSC/EPC group. Upregulation was strongest in the MSC/EPC/HSC co-cultures. There were no significant changes in ALP levels and calcium content, but ALP activity was slightly higher and calcium content was relatively lower in the MSC/EPC and MSC/EPC/HSC groups.

CONCLUSIONS

Co-culture of MSCs with HSCs or EPCs/HSCs upregulated the expression of osteogenesis-related genes but did not affect the efficiency of osteogenesis.

摘要

目的

骨组织工程在广泛应用于临床之前，成骨和血管生成的效率面临着需要克服的挑战。我们旨在优化一种培养系统以增强成骨和血管生成。我们研究了造血干细胞（HSCs）与间充质干细胞（MSCs）和内皮祖细胞（EPCs）共培养时是否能促进成骨。

方法

将MSC/HSC、MSC/EPC/HSC和MSC/EPC共培养物孵育21天。分析碱性磷酸酶（ALP）活性和钙含量以评估矿化情况。在第28天通过测量mRNA水平来评估编码成骨相关蛋白（碱性磷酸酶（）、I型胶原（）、骨钙素（）和骨桥蛋白（））的基因表达水平。通过管形成试验评估血管生成。

结果

与MSC/EPC组相比，MSC/HSC和MSC/EPC/HSC共培养物中 、 、 和 基因上调。在MSC/EPC/HSC共培养物中上调最为明显。ALP水平和钙含量无显著变化，但MSC/EPC和MSC/EPC/HSC组的ALP活性略高，钙含量相对较低。

结论

MSCs与HSCs或EPCs/HSCs共培养上调了成骨相关基因的表达，但不影响成骨效率。

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