Blackburn Michael B, Farrar Robert R, Sparks Michael E, Kuhar Daniel, Mitchell Ashaki, Gundersen-Rindal Dawn E
Invasive Insect Biocontrol and Behavior Laboratory, USDA, ARS, Bldg 007, Rm 301, BARC-West, Beltsville, MD 20705, USA.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3417-3422. doi: 10.1099/ijsem.0.002127. Epub 2017 Aug 22.
Sixteen isolates of Gram-reaction-negative, motile, violet-pigmented bacteria were isolated from Sphagnum bogs in West Virginia and Maine, USA. 16S rRNA gene sequences and fatty acid analysis revealed a high degree of relatedness among the isolates, and genome sequencing of two isolates, IIBBL 14B-1T and IIBBL 37-2 (from West Virginia and Maine, respectively), revealed highly similar genomic sequences. The average nucleotide identity (gANI) calculated for these two isolates was found to be in excess of 99 %, but did not exceed 88 % when comparing either isolate with genomic sequences of Chromobacterium violaceum ATCC 12472T, C. haemolyticum DSM 19808T, C. piscinae ND17, C. subtsugae PRAA4-1T, C. vaccinii MWU205T or C. amazonense CBMAI 310T. Collectively, gANI and 16S rRNA gene sequence comparisons suggested that isolates IIBBL 14B-1T and IIBBL 37-2 were most closely related to C. subtsugae, but represented a distinct species. We propose the name Chromobacterium sphagni sp. nov. for this taxon; the type strain is IIBBL 14B-1T (=NRRL B-67130T=JCM 31882T).
从美国西弗吉尼亚州和缅因州的泥炭藓沼泽中分离出16株革兰氏阴性、具运动性、产紫色色素的细菌。16S rRNA基因序列和脂肪酸分析表明这些分离株之间具有高度相关性，对两株分离株IIBBL 14B-1T和IIBBL 37-2（分别来自西弗吉尼亚州和缅因州）进行全基因组测序，结果显示它们的基因组序列高度相似。计算得出这两株分离株的平均核苷酸一致性（gANI）超过99%，但将其中任何一株与紫色色杆菌ATCC 12472T、溶血色杆菌DSM 19808T、鱼色杆菌ND17、杉木叶色杆菌PRAA4-1T、越橘色杆菌MWU205T或亚马逊色杆菌CBMAI 310T的基因组序列进行比较时，gANI均不超过88%。综合gANI和16S rRNA基因序列比较结果表明，分离株IIBBL 14B-1T和IIBBL 37-2与杉木叶色杆菌关系最为密切，但代表一个独特的物种。我们提议将这个分类单元命名为泥炭藓色杆菌（Chromobacterium sphagni sp. nov.）；模式菌株为IIBBL 14B-1T（=NRRL B-67130T=JCM 31882T）。
