贻贝鳃中纤毛摆动频率的控制——II. 皂苷和Brij - 58对侧纤毛的影响。

Control of ciliary beat frequency in the gill of Mytilus--II. Effects of saponin and Brij-58 on the lateral cilia.

作者信息

Murakami A

出版信息

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1987;86(2):281-7. doi: 10.1016/0742-8413(87)90080-6.

Abstract

The effects of saponin and Brij-58 on the beat activation of the lateral cilia on the gill of Mytilus edulis were investigated. The ciliary activation by 5-hydroxytryptamine (5HT) decreased as the saponin-induced permeabilization progressed, increasing the reactivation of the ciliary beat by extracellularly applied ATP (1 mM). The cilia were activated by 5HT even after the treatment with saponin (0.01 and 0.02% w/v) or Brij-58 (0.07%) rendered the preparation capable of the reactivation by ATP. The saponin treatment itself stimulated the beat of the cilia. Theophylline (1 mM) augmented the saponin-induced activation of the cilia.

摘要

研究了皂苷和Brij - 58对紫贻贝鳃外侧纤毛搏动激活的影响。随着皂苷诱导的通透性增加，5 - 羟色胺（5HT）引起的纤毛激活减少，细胞外施加ATP（1 mM）可增加纤毛搏动的再激活。在用皂苷（0.01%和0.02% w/v）或Brij - 58（0.07%）处理使制剂能够被ATP再激活后，纤毛仍能被5HT激活。皂苷处理本身会刺激纤毛的搏动。茶碱（1 mM）增强了皂苷诱导的纤毛激活。