Tanideh N, Zare Z, Jamshidzadeh A, Lotfi M, Azarpira Negar, Sepehrimanesh M, Koohi-Hosseinabadi O
a Stem cell and Transgenic Technology Research Center.
b Department of Pharmacology , School of Medicine.
Biotech Histochem. 2017;92(6):417-424. doi: 10.1080/10520295.2017.1308013. Epub 2017 Aug 22.
We investigated the therapeutic effects of an extract of Psidium guajava (guava) leaf on experimentally induced osteoarthritis in guinea pig. The left knee of 30 male guinea pigs was anesthetized and the cranial cruciate ligament was severed. The animals were followed for 8 weeks until osteoarthritis was confirmed by radiography and histopathology. Animals were divided randomly into five groups; group 1, the ligament was severed and untreated; group 2, the ligament was severed and treated with piascledine, an extract of soybean and avocado; group 3, the ligament was severed and treated with 200 mg/kg hydroethanolic extract of guava; group 4, the ligament was severed and treated with 400 mg/kg hydroethanolic extract of guava; and group 5, control animals without surgery or extracts. Radiological and histopathological evaluations after 8 weeks showed reduced severity of osteoarthritis in the piascledine treatment group compared to group 1. The guava extract also reduce the severity of osteoarthritis compared to controls. Histopathological examination of treatment and control groups showed that treatment the guava extract improved lesions significantly. Hydroethanolic extracts of guava leaf appears to prevent osteoarthritis by inhibition of free radical formation in the knee joint.
我们研究了番石榴叶提取物对实验性诱导的豚鼠骨关节炎的治疗效果。对30只雄性豚鼠的左膝进行麻醉，切断颅交叉韧带。对这些动物进行8周的跟踪观察，直到通过放射学和组织病理学确认骨关节炎。将动物随机分为五组；第1组，切断韧带且不进行治疗；第2组，切断韧带并用碧萝芷（一种大豆和鳄梨的提取物）治疗；第3组，切断韧带并用200mg/kg番石榴水乙醇提取物治疗；第4组，切断韧带并用400mg/kg番石榴水乙醇提取物治疗；第5组，为未进行手术或未接受提取物的对照动物。8周后的放射学和组织病理学评估显示，与第1组相比，碧萝芷治疗组骨关节炎的严重程度降低。与对照组相比，番石榴提取物也降低了骨关节炎的严重程度。治疗组和对照组的组织病理学检查表明，番石榴提取物治疗显著改善了病变。番石榴叶的水乙醇提取物似乎通过抑制膝关节中的自由基形成来预防骨关节炎。
