Walton D G, Acton A B, Stich H F
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1987;86(2):399-404. doi: 10.1016/0742-8413(87)90103-4.
Unscheduled DNA repair synthesis was measured autoradiographically in cultured rainbow trout gonad (RTG) and human fibroblast (HF) cells following exposure to aflatoxin B1 (AFB1), 3,4-benzopyrene (BP), 1,2,5,6-dibenzanthracene (DBA), 1,2-benzanthracene (BA) and pyrene (PY) activated with S9 prepared from rainbow trout liver. S9 from rainbow trout injected with Arochlor 1254 or an oil extract was compared with S9 from Fischer rats injected with Arochlor 1254 for the ability to activate AFB1 and cause DNA repair in RTG and HF cells. All three types of S9 activated AFB1, but the measured DNA repair response was greater in the HF cells. A significant grain count response was found following exposure of HF cells to fish S9-activated BP. Using assay conditions which enhance fish cell grain counts, a significant level of DNA repair was also found in RTG cells exposed to fish S9-activated BP. Marginal but statistically significant amounts of DNA repair were elicited in HF and RTG cells exposed to rainbow trout S9-activated BA and DBA, but no response was detected following PY exposure. Fish S9 was found to be able to activate a series of polycyclic aromatic hydrocarbons (PAH) and cause DNA repair synthesis in both fish and mammalian cells. The magnitude of the repair response roughly parallels the carcinogenic potential of the PAHs. These results elicit trans species and phyla comparisons which help to validate fish as models for aquatic carcinogenesis research, and also demonstrate PAH DNA-damaging effects on fish DNA, adding further credence for studying the effects of these chemicals on aquatic organisms.
在用虹鳟鱼肝制备的S9激活黄曲霉毒素B1（AFB1）、3,4-苯并芘（BP）、1,2,5,6-二苯并蒽（DBA）、1,2-苯并蒽（BA）和芘（PY）后，通过放射自显影法测定培养的虹鳟性腺（RTG）和人成纤维细胞（HF）中的非程序性DNA修复合成。将注射了多氯联苯混合物1254或油提取物的虹鳟的S9与注射了多氯联苯混合物1254的Fischer大鼠的S9在激活AFB1并在RTG和HF细胞中引起DNA修复的能力方面进行了比较。所有三种类型的S9都能激活AFB1，但在HF细胞中测得的DNA修复反应更强。在HF细胞暴露于鱼S9激活的BP后发现有显著的颗粒计数反应。使用增强鱼细胞颗粒计数的检测条件，在暴露于鱼S9激活的BP的RTG细胞中也发现了显著水平的DNA修复。在暴露于虹鳟S9激活的BA和DBA的HF和RTG细胞中引发了少量但具有统计学意义的DNA修复，但在PY暴露后未检测到反应。发现鱼S9能够激活一系列多环芳烃（PAH）并在鱼类和哺乳动物细胞中引起DNA修复合成。修复反应的程度大致与PAHs的致癌潜力平行。这些结果引发了跨物种和跨门类的比较，有助于验证鱼类作为水生致癌作用研究模型的有效性，同时也证明了PAH对鱼类DNA的损伤作用，进一步证明了研究这些化学物质对水生生物影响的可信度。
