McPhillips D M, Kalin J R, Hill D L
Drug Metab Dispos. 1987 Mar-Apr;15(2):207-11.
An improved method for the analysis of retinoids in mouse plasma allows quantification of all-trans-retinoic acid (RA) and N-(2-hydroxyethyl)retinamide (NHERA) in amounts as low as 2 ng/ml (7 X 10(-9) M), the approximate endogenous concentration of RA. The procedure, involving solid-phase extraction and reverse-phase HPLC, can be used for rapid processing of large numbers of samples. With this procedure, we have determined the terminal half-life for RA (0.5 hr) and have confirmed the half-life for NHERA (3.6 hr) in the plasma of mice dosed orally. We have also noted that the serum content of retinol is temporarily reduced following dosing with RA, but not with NHERA.
一种改进的小鼠血浆中类视黄醇分析方法能够定量低至2 ng/ml（7×10⁻⁹ M）的全反式维甲酸（RA）和N-(2-羟乙基)视黄酰胺（NHERA），这一浓度近似于RA的内源性浓度。该方法包括固相萃取和反相高效液相色谱，可用于大量样品的快速处理。通过此方法，我们测定了口服给药小鼠血浆中RA的终末半衰期为0.5小时，并确认了NHERA的半衰期为3.6小时。我们还注意到，给予RA后，血清视黄醇含量会暂时降低，但给予NHERA后则不会。
