Jorge L F, Meniqueti A B, Silva R F, Santos K A, Da Silva E A, Gonçalves J E, De Rezende C M, Colauto N B, Gazim Z C, Linde G A
Programa de Pós-Graduação em Biotecnologia Aplicada à Agricultura, Universidade Paranaense, Umuarama, PR, Brasil.
Centro de Tecnologia, Instituto de Química, Universidade Federal do Rio de Janeiro, Cidade Universitária, , Brasil.
Genet Mol Res. 2017 Aug 17;16(3):gmr-16-03-gmr.16039714. doi: 10.4238/gmr16039714.
In this study, the temperature and pressure of supercritical CO extraction were evaluated to obtain oleoresin of Brunfelsia uniflora leaves and flowers. The oleoresin compounds were identified by gas chromatography-mass spectrometry. The antioxidant activity was evaluated by three different methods. The highest oleoresin yields were 3.32% at 40°C and 200 bar for the leaves, and 1.03% at 60°C and 200 bar for the flowers. The main extracted compounds from leaves were phytol varying from 11.95 to 36.42% and α-tocopherol from 15.53 to 43.10%, and from flowers were geranyl linalool from 11.05 to 21.42% and α-amyrin from 9.66 to 22.12%. Oleoresin obtained at 60°C and 150 bar from leaves presented high antioxidant activity by DPPH (IC 1.90 mg/mL) and by FRAP (1.8 µmol Fe/mg). β-carotene/linoleic acid co-oxidation oleoresin from leaves at 0.25 mg/mL presented higher antioxidant activity than Trolox. The total phenolic content of the oleoresin from leaves ranged from 66.20 to 83.33 µg/mg and from flowers it was just up to 12.46 µg/mg. The extraction conditions affected yield, chemical composition, and antioxidant activity of oleoresin from leaves and flowers. This is the first report on the antioxidant activity of B. uniflora oleoresin from leaves and flowers and provides subsidies for potential applications in chemical, pharmaceutical, and food industries.
在本研究中，对超临界CO₂萃取的温度和压力进行了评估，以获取单花鸳鸯茉莉叶和花的油树脂。通过气相色谱 - 质谱联用仪鉴定油树脂化合物。采用三种不同方法评估抗氧化活性。叶在40°C和200 bar条件下油树脂得率最高，为3.32%；花在60°C和200 bar条件下油树脂得率最高，为1.03%。叶中主要提取化合物为叶绿醇，含量在11.95%至36.42%之间，α - 生育酚含量在15.53%至43.10%之间；花中主要提取化合物为香叶基芳樟醇，含量在11.05%至21.42%之间，α - 香树脂醇含量在9.66%至22.12%之间。叶在60°C和150 bar条件下获得的油树脂通过DPPH法（IC₅₀为1.90 mg/mL）和FRAP法（1.8 µmol Fe/mg）呈现出高抗氧化活性。叶中0.25 mg/mL的β - 胡萝卜素/亚油酸共氧化油树脂的抗氧化活性高于Trolox。叶中油树脂的总酚含量在66.20至83.33 µg/mg之间，花中油树脂的总酚含量仅为12.46 µg/mg。萃取条件影响叶和花中油树脂的得率、化学成分及抗氧化活性。这是关于单花鸳鸯茉莉叶和花油树脂抗氧化活性的首次报道，为其在化学、制药和食品工业中的潜在应用提供了依据。
