Lagares M H, Silva K S F, Barbosa A M, Rodrigues D A, Costa I R, Martins J V M, Morais M P, Campedelli F L, Moura K K V O
Núcleo de Pesquisa Replicon, , , Brasil.
Departamento de Biomedicina, , , Brasil.
Genet Mol Res. 2017 Aug 17;16(3):gmr-16-03-gmr.16039721. doi: 10.4238/gmr16039721.
Atherosclerosis is a multifactorial pathological disease that alters the morphology and function of arterial walls. The atheroma growth leads to vessel hardening and lumen narrowing, limiting the blood flow. The atheroma plaque can eventually break, expose highly thrombogenic material and lead to platelet activation and subsequent formation of a thrombus that may block blood flow in loco, or even leading to obstruction of other vessels with a smaller diameter. This process is one of the main determinants of the clinical manifestations of atherosclerosis, such as coronary artery disease, ischemic stroke, and peripheral arterial disease. Although the inflammatory theory about atherosclerosis is the most renowned one, observations point to common biological characteristics between cancer and atherosclerosis suggesting a possible association between p53 and atherosclerotic diseases. We collected peripheral blood samples from 200 individuals with clinical manifestations of atherosclerotic disease and 100 individuals without manisfestation of the disease to form the control group. DNA was subjected to molecular analysis (PCR) to identify the polymorphism of the p53 gene. We have not found any relationship between the polymorphism of the p53 gene and atherosclerosis in the population studied (P = 0.36). There was no relationship between atherosclerosis, polymorphism of p53 and the variables accounted: smoking habit (P = 0.72, 0.51 and 0.62 for smokers, non-smokers and former smokers respectively), alcohol consumption (P = 0.17 for individuals with drinking habits and 0.38 for those who do not consume alcohol beverage), systemic arterial hypertension (P = 0.60), diabetes mellitus (P = 0.34), and dyslipidemia (P = 0.89). Our population has a high rate of miscegenation and heterozygotes, and according to studies the arginine variant is more related to plaque formation because it induces apoptosis more frequently when compared to the proline variant. According to our results, there is no association between the polymorphism of the p53 gene, atherosclerosis and its risk factors in the population studied.
动脉粥样硬化是一种多因素的病理疾病，它会改变动脉壁的形态和功能。动脉粥样瘤的生长会导致血管硬化和管腔狭窄，从而限制血液流动。动脉粥样瘤斑块最终可能破裂，暴露具有高度血栓形成性的物质，导致血小板活化，并随后形成血栓，这可能会局部阻塞血流，甚至导致直径较小的其他血管阻塞。这个过程是动脉粥样硬化临床表现的主要决定因素之一，如冠状动脉疾病、缺血性中风和外周动脉疾病。尽管关于动脉粥样硬化的炎症理论最为著名，但观察结果表明癌症和动脉粥样硬化之间存在共同的生物学特征，这表明p53与动脉粥样硬化疾病之间可能存在关联。我们从200名有动脉粥样硬化疾病临床表现的个体和100名无该疾病表现的个体中采集外周血样本，以形成对照组。对DNA进行分子分析（PCR）以鉴定p53基因的多态性。在我们研究的人群中，未发现p53基因多态性与动脉粥样硬化之间存在任何关系（P = 0.36）。动脉粥样硬化、p53多态性与所考虑的变量之间也没有关系：吸烟习惯（吸烟者、非吸烟者和曾经吸烟者的P值分别为0.72、0.51和0.62）、饮酒情况（有饮酒习惯者的P值为0.17，不饮酒者的P值为0.38）、系统性动脉高血压（P = 0.60）、糖尿病（P = 0.34）和血脂异常（P = 0.89）。我们的人群有很高的混血率和杂合子，根据研究，精氨酸变体与斑块形成的关系更大，因为与脯氨酸变体相比，它更频繁地诱导细胞凋亡。根据我们的结果，在我们研究的人群中，p53基因多态性、动脉粥样硬化及其危险因素之间不存在关联。
