Zalesak Michal, Ruzicka Jan, Vicha Robert, Dvorackova Marie
Department of Environment Protection Engineering, Tomas Bata University in Zlin, Faculty of Technology, Vavreckova 275, 760 01, Zlin, Czech Republic.
Department of Chemistry, Tomas Bata University in Zlin, Faculty of Technology, Vavreckova 275, 760 01, Zlin, Czech Republic.
Chemosphere. 2017 Nov;186:919-927. doi: 10.1016/j.chemosphere.2017.07.156. Epub 2017 Jul 31.
An environmental isolate Comamonas testosteroni strain RF2, which has been found to cometabolize trichloroethene (TCE) in the presence of phenol and sodium lactate as growth substrates, was tested to investigate its capacity for degrading 1,2-cis-dichloroethene (cDCE), 1,2-trans-dichlorothene (tDCE), and 1,1-dichloroethene (1,1DCE). Degradation assays were performed for single DCEs, as well as for a mixture of DCEs with TCE, which resembled contaminated plume in groundwater. RF2 was capable of efficiently removing all three dichloroethenes (DCEs) at the initial aqueous concentrations of 6.01 mg L for cDCE, 3.80 mg L for tDCE and 0.65 mg L for 1,1DCE, with a removal efficiency of 100% for cDCE, 65.8% for tDCE, and 46.8% for 1,1DCE. Furthermore, complete removal of TCE, cDCE and 1,1DCE (122.5 μg L, 84.3 μg L and 51.4 μg L, respectively) was observed in a mixture sample that also contained 72.33 μg L of tDCE, which was removed to the amount of 72.3%. Moreover, degradation of cDCE (6.01 mg L) led to a 93.8% release of inorganic chloride, and 2,2-dichloroacetaldehyde was determined as the first intermediate of cDCE transformation. The findings of this study suggest that the strain RF2 exhibits the potential to remediate groundwater contaminated with less chlorinated ethenes.
一种环境分离物睾丸酮丛毛单胞菌菌株RF2，已发现在以苯酚和乳酸钠作为生长底物的情况下能共代谢三氯乙烯（TCE），对其降解1,2-顺式二氯乙烯（cDCE）、1,2-反式二氯乙烯（tDCE）和1,1-二氯乙烯（1,1DCE）的能力进行了测试。对单一二氯乙烯以及二氯乙烯与TCE的混合物进行了降解试验，该混合物类似于地下水中的污染羽流。RF2能够在初始水浓度为cDCE 6.01 mg/L、tDCE 3.80 mg/L和1,1DCE 0.65 mg/L的情况下有效去除所有三种二氯乙烯，cDCE的去除效率为100%，tDCE为65.8%，1,1DCE为46.8%。此外，在一个还含有72.33 μg/L tDCE的混合样品中，观察到TCE、cDCE和1,1DCE（分别为122.5 μg/L、84.3 μg/L和51.4 μg/L）被完全去除，tDCE被去除至72.3%。此外，cDCE（6.01 mg/L）的降解导致93.8%的无机氯释放，并且确定2,2-二氯乙醛是cDCE转化的首个中间产物。本研究结果表明，菌株RF2具有修复受低氯代乙烯污染地下水的潜力。
