Department of Environment Protection Engineering, Tomas Bata University in Zlin, Faculty of Technology, Vavreckova 275, 760 01, Zlin, Czech Republic.
Department of Chemistry, Tomas Bata University in Zlin, Faculty of Technology, Vavreckova 275, 760 01, Zlin, Czech Republic.
Chemosphere. 2021 Apr;269:128770. doi: 10.1016/j.chemosphere.2020.128770. Epub 2020 Oct 27.
An environmental isolate Comamonas testosteroni RF2 has been previously described to cometabolize trichloroethene (TCE), 1,2-cis-dichloroethene (cDCE), 1,2-trans-dichloroethene (tDCE), and 1,1-dichloroethene (1,1DCE) when grown on phenol and lactate sodium. In this study, three vinyl chloride (VC) degrading strains, Mycobacterium aurum L1, Pseudomonas putida PS, and Rhodococcus ruber Sm-1 were used to form consortia with the strain RF2 in terms to achieve the removal of VC along with above-mentioned chloroethenes. Degradation assays were performed for a binary mixture of cDCE and VC as well as for a mixture of TCE, all DCEs and VC. The consortium composed of C. testosteroni RF2 and M. aurum L1 showed to be the most efficient towards the removal of cDCE (6.01 mg L) in the binary mixture with VC (10 mg L) and was capable of efficiently removing chloroethenes in the mixture sample at the initial concentrations of 116 μg L for TCE, 662 μg L for cDCE, 42 μg L for tDCE, 16 μg L for 1,1DCE, and 7 mg L for VC with a removal efficiency of nearly 100% for all of the compounds. Although complete removal of VC took a significantly longer time than the removal of other chloroethenes, the consortium composed of C. testosteroni RF2 and M. aurum L1 displayed strong bioremediation potential for aquifers with downstream contamination characterized by the presence of less chlorinated ethenes.
先前有研究报道，从环境中分离得到的罗托考沙雷氏菌（Comamonas testosteroni）RF2 能够以苯酚和乳酸钠为共基质，共代谢三氯乙烯（TCE）、1,2-顺式二氯乙烯（cDCE）、1,2-反式二氯乙烯（tDCE）和 1,1-二氯乙烯（1,1DCE）。本研究中，我们利用三种氯乙烯（VC）降解菌，即金黄分枝杆菌（Mycobacterium aurum L1）、恶臭假单胞菌（Pseudomonas putida PS）和红平红球菌（Rhodococcus ruber Sm-1），与菌株 RF2 形成共代谢菌系，以实现 VC 与上述氯代烯烃的共代谢去除。我们分别进行了 cDCE 和 VC 的二元混合物以及 TCE、所有 DCE 和 VC 的混合物的降解实验。结果表明，在含有 10mg/L VC 的 cDCE 和 VC 的二元混合物中，C. testosteroni RF2 和 M. aurum L1 组成的共代谢菌系对 cDCE（6.01mg/L）的去除效果最为显著，并且在初始浓度为 116μg/L 的 TCE、662μg/L 的 cDCE、42μg/L 的 tDCE、16μg/L 的 1,1DCE 和 7mg/L 的 VC 的混合物样品中，该菌系也能有效地去除所有氯代烯烃，去除率接近 100%。虽然 VC 的完全去除比其他氯代烯烃的去除需要更长的时间，但 C. testosteroni RF2 和 M. aurum L1 组成的共代谢菌系具有很强的生物修复潜力，可用于下游存在低氯代烯烃污染的含水层。
