Bak Gye-Ryeong, Lee Kiseok Keith, Clark Ian M, Mauchline Tim H, Kavamura Vanessa Nessner, Jee Samnyu, Lee Jeong-Tae, Kim Hyun, Lee Yong-Hwan
Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration, Pyeongchang, 25342, Republic of Korea.
Department of Agricultural Biotechnology, Seoul National University, Seoul, 08826, Republic of Korea.
Sci Rep. 2025 Jan 17;15(1):2284. doi: 10.1038/s41598-025-86944-6.
Plant root and soil-associated microbiomes are influenced by niches, including bulk and rhizosphere soil. In this work, we collected bulk and rhizosphere soil samples at four potato developmental stages (leaf growth, flowering, tuber elongation and harvest) to identify whether rhizosphere microbiota are structured in a growth stage-dependent manner. The bacterial and fungal microbiota showed significant temporal differences in the rhizosphere and bulk soil. Rhizobacteria were most diverse at the tuber elongation stage, and dominant ASVs identified as Sphingomonas, Rhodanobacter, Sphingobium, Hyphomicrobium, and Solirubrobacter spp. In contrast, rhizosphere fungal diversity peaked at flowering stage, with Lecanicillium spp. being prominent. Furthermore, the abundance of saprophytic fungal genera, including Colletotrichum and Fusarium, and Alternaria, sharply increased at harvest stage, likely contributing to plant residue decomposition. Indicator taxa analysis highlighted the dominance of these genera at harvest. Network analysis revealed increased microbial complexity during the later growth stage, with 721 edges compared to 521 edges in the early growth stage. This increase included positive correlations between bacteria and negative correlations between bacteria and fungi. These changes suggest that microbial interactions become more interconnected and complex as potato plants mature. Our findings highlight the potential role of saprophytic fungi in shaping microbial dynamics during the later growth stage in rhizosphere soil.
植物根系和与土壤相关的微生物群落受到生态位的影响，包括土体和根际土壤。在这项研究中，我们在马铃薯的四个发育阶段（叶片生长、开花、块茎伸长和收获）采集了土体和根际土壤样本，以确定根际微生物群是否以生长阶段依赖的方式构建。细菌和真菌微生物群在根际和土体土壤中表现出显著的时间差异。根际细菌在块茎伸长阶段最为多样，优势扩增子序列变体（ASVs）被鉴定为鞘氨醇单胞菌属、红杆菌属、鞘脂单胞菌属、生丝微菌属和嗜油杆菌属。相比之下，根际真菌多样性在开花阶段达到峰值，其中莱氏绿僵菌属较为突出。此外，包括炭疽菌属、镰刀菌属和链格孢属在内的腐生真菌属的丰度在收获阶段急剧增加，可能有助于植物残体的分解。指示类群分析突出了这些属在收获时的优势地位。网络分析显示，在生长后期微生物复杂性增加，后期有721条边，而早期生长阶段有521条边。这种增加包括细菌之间的正相关以及细菌与真菌之间的负相关。这些变化表明，随着马铃薯植株成熟，微生物相互作用变得更加相互关联和复杂。我们的研究结果突出了腐生真菌在塑造根际土壤后期生长阶段微生物动态中的潜在作用。
