Nisbet Lorna A, Venson Rafael, Wylie Fiona M, Scott Karen S
Department of Forensic Medicine and Science, University of Glasgow, Glasgow, G12 8QQ, UK.
Department of Forensic Science, Arcadia University, 450 S Easter Road, Glenside, PA 19038, USA.
J Anal Toxicol. 2017 Jul 1;41(6):559-565. doi: 10.1093/jat/bkx053.
NBOMes are a group of new psychoactive substances derived from phenethylamines. Recreational abuse is thought to have begun in 2010 and they are commonly associated with the "club drug" scene. They are administered in liquid form or as blotters due to their high potency. An LC-MS-MS method was validated using Scientific Working Group for Forensic Toxicology parameters for the detection of 25B-, 25C- and 4-iodo-2,5-dimethoxy-N-[(2-methoxyphenyl)methyl]-benzeneethanamine (25I-NBOMe) using 4-bromo-2,5-dimethoxy-N-[(2-methoxyphenyl)methyl]-benzeneethanamine (25B-NBOMe)-D3 as internal standard for urine and hair. Calibration graphs with R2 values >0.99 were observed for urine and hair for concentrations ranging from 0.1 to 100 ng/mL and 0.025 to 2.5 ng/mg, respectively. Urine LODs ranged from 5 to 25 pg/mL and had an LOQ of 50 pg/mL. Hair LOD and LOQs ranged from 3 to 5 pg/mg and 6.25 to 12.5 pg/mg, respectively. Intra- and inter-day precision was <20% and accuracy was within ±20% for both matrices. The method was shown to be selective for both exogenous and endogenous compounds. No matrix effects were observed for either matrix. LLE recovery ranged from 90 to 103% for urine samples and solid phase extraction recovery ranged from 80 to 107% for hair samples. Long-Evans rats (n = 55) were administered 25B-, 25C- or 25I-NBOMe at doses ranging from 30 to 300 μg/kg over a period of 10 days. Rats were shaved prior to their first dose and re-shaved after the 10-day period. Hair was separated by color (black: n = 55 and white: n = 55) and analyzed using the validated LC-MS-MS method to assess the impact hair color has on the incorporation of these drugs. All drugs were successfully detected in black hair. 25B-NBOMe from rats receiving the highest dose and 25C-NBOMe from rats receiving the medium and high doses were quantified in white hair. 25I-NBOMe was detected but fell below the limit of quantification. A dose-dependent concentration increase was observed in the black hair. All pooled urine samples tested positive for their expected NBOMes.
N-苄基苯乙胺类物质（NBOMes）是一类从苯乙胺衍生而来的新型精神活性物质。据信，其娱乐性滥用始于2010年，并且它们通常与“俱乐部毒品”场景相关联。由于其高效力，它们以液体形式或吸墨纸形式给药。使用法医毒理学科学工作组的参数验证了一种液相色谱-串联质谱（LC-MS-MS）方法，该方法以4-溴-2,5-二甲氧基-N-[(2-甲氧基苯基)甲基]苯乙胺（25B-NBOMe）-D3作为内标，用于检测尿液和毛发中的25B-、25C-和4-碘-2,5-二甲氧基-N-[(2-甲氧基苯基)甲基]苯乙胺（25I-NBOMe）。对于尿液和毛发，分别在浓度范围为0.1至100 ng/mL和0.025至2.5 ng/mg时观察到相关系数（R2）值>0.99的校准曲线。尿液的检测限（LOD）范围为5至25 pg/mL，定量限（LOQ）为50 pg/mL。毛发的LOD和LOQ分别为3至5 pg/mg和6.25至12.5 pg/mg。两种基质的日内和日间精密度均<20%，准确度在±20%以内。该方法对外源性和内源性化合物均具有选择性。两种基质均未观察到基质效应。尿液样本的液液萃取（LLE）回收率为90%至103%，毛发样本的固相萃取回收率为80%至107%。对55只Long-Evans大鼠在10天内给予剂量范围为30至300 μg/kg的25B-、25C-或25I-NBOMe。大鼠在首次给药前剃毛，并在10天周期后再次剃毛。将毛发按颜色分开（黑色：n = 55，白色：n = 55），并使用经过验证的LC-MS-MS方法进行分析，以评估毛发颜色对这些药物掺入的影响。在黑色毛发中成功检测到所有药物。在接受最高剂量的大鼠的白色毛发中定量检测到25B-NBOMe，在接受中高剂量的大鼠的白色毛发中定量检测到25C-NBOMe。检测到了25I-NBOMe，但低于定量限。在黑色毛发中观察到剂量依赖性的浓度增加。所有合并的尿液样本对预期的NBOMes检测均呈阳性。
