Chen Guangxin, Gao Zhenhua, Chu Wenhui, Cao Zan, Li Chunyi, Zhao Haiping
State Key Laboratory of Special Animal Molecular Biology, Changchun 130112, China.
Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, China.
Asian-Australas J Anim Sci. 2018 Apr;31(4):569-575. doi: 10.5713/ajas.17.0289. Epub 2017 Aug 22.
This experiment was conducted to investigate the effects of chromium picolinate (CrP) on fat deposition, genetic expression and enzymatic activity of lipid metabolism-related enzymes.
Two hundred forty one-day-old Ross broilers were randomly divided into 5 groups with 4 replicates per group and 12 Ross broiler chicks per replicate. The normal control group was fed a basal diet, and the other groups fed the same basal diet supplemented with 0.1, 0.2, 0.4, and 0.8 mg/kg CrP respectively. The experiment lasted for 21 days.
Added CrP in the basal diet decreased the abdominal fat, had no effects on subcutaneous fat thickness and inter-muscular fat width; 0.2 mg/kg CrP significantly decreased the fatty acid synthase (FAS) enzymatic (p<0.05); acetyl-CoA carboxylase (ACC) enzymatic activity decreased in all CrP groups (p<0.05); hormone-sensitive lipase (HSL) enzymatic activity also decreased, but the change was not significant (p>0.05); 0.4 mg/kg CrP group significantly decreased the lipoprotein lipase (LPL) enzymatic activity. FAS mRNA expression increased in all experimental groups, and the LPL mRNA expression significantly increased in all experimental groups (p<0.05), but not 0.2 mg/kg CrP group.
The results indicated that adding CrP in basal diet decreased the abdominal fat percentage, had no effects on subcutaneous fat thickness and inter-muscular fat width, decreased the enzymatic activity of FAS, ACC, LPL and HSL and increased the genetic expression levels of FAS and LPL.
本试验旨在研究吡啶甲酸铬（CrP）对脂肪沉积、脂质代谢相关酶的基因表达及酶活性的影响。
选取240只1日龄罗斯肉鸡，随机分为5组，每组4个重复，每个重复12只鸡。正常对照组饲喂基础日粮，其他组在基础日粮中分别添加0.1、0.2、0.4和0.8 mg/kg的CrP。试验期为21天。
基础日粮中添加CrP可降低腹脂，对皮下脂肪厚度和肌间脂肪宽度无影响；0.2 mg/kg CrP显著降低脂肪酸合酶（FAS）活性（p<0.05）；所有添加CrP组的乙酰辅酶A羧化酶（ACC）活性均降低（p<0.05）；激素敏感脂肪酶（HSL）活性也降低，但变化不显著（p>0.05）；0.4 mg/kg CrP组显著降低脂蛋白脂肪酶（LPL）活性。所有试验组FAS mRNA表达均升高，所有试验组LPL mRNA表达均显著升高（p<0.05），但0.2 mg/kg CrP组除外。
结果表明，基础日粮中添加CrP可降低腹脂率，对皮下脂肪厚度和肌间脂肪宽度无影响，降低FAS、ACC、LPL和HSL的酶活性，提高FAS和LPL的基因表达水平。
