State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , 5625 Renmin Street, Changchun, Jilin 130022, People's Republic of China.
University of Chinese Academy of Sciences , Beijing 100049, People's Republic of China.
Anal Chem. 2017 Sep 19;89(18):9864-9869. doi: 10.1021/acs.analchem.7b01939. Epub 2017 Sep 8.
Electrogenerated chemiluminescence (ECL) application of stainless steel, a robust and cost-effective material, has been developed for the first time. Type 304 stainless steel electrode shows appealing ECL performance in the luminol-HO system. It enables the detection of HO with a linear range from 1 to 1000 nM and a limit of detection of 0.456 nM [signal-to-noise ratio (S/N) = 3]. The ECL method based on type 304 stainless steel electrode is more sensitive, more cost-effective, and much simpler than other ECL methods reported before. Because the stainless steel electrode has excellent performance for HO detection and HO participates in many important enzymatic reactions, applications of stainless steel electrode-based ECL for detection of enzyme activities and enzyme substrates were further investigated by use of glucose oxidase (GODx) and glucose as representative enzyme and substrate. The concentrations of glucose and the activity of GODx were directly proportional to ECL intensities over a range of 0.1-1000 μM and 0.001-0.7 units/mL with limits of detection of 0.076 μM and 0.00087 unit/mL (S/N = 3), respectively. This method was successfully used for determining glucose in honey. Because of their remarkable performance and user-friendly features, stainless steel electrodes hold great promise in various electroanalytical applications, such as biosensing, disposable sensors, and wearable sensors.
首次开发了一种基于不锈钢的电化学发光（ECL）应用，不锈钢是一种坚固且具有成本效益的材料。304 型不锈钢电极在鲁米诺-HO 体系中表现出诱人的 ECL 性能。它能够检测到 HO，线性范围为 1 到 1000 nM，检测限为 0.456 nM [信噪比（S/N）= 3]。基于 304 型不锈钢电极的 ECL 方法比以前报道的其他 ECL 方法更灵敏、更具成本效益、更简单。由于不锈钢电极对 HO 检测具有优异的性能，并且 HO 参与许多重要的酶反应，因此进一步研究了基于不锈钢电极的 ECL 用于检测酶活性和酶底物的应用，使用葡萄糖氧化酶（GODx）和葡萄糖作为代表性酶和底物。葡萄糖和 GODx 的浓度与 ECL 强度成正比，范围为 0.1-1000 μM 和 0.001-0.7 单位/mL，检测限分别为 0.076 μM 和 0.00087 单位/mL（S/N = 3）。该方法成功用于测定蜂蜜中的葡萄糖。由于其出色的性能和用户友好的特点，不锈钢电极在各种电化学生物传感器、一次性传感器和可穿戴传感器等应用中具有广阔的应用前景。
