Xu Shiquan, Su Zhaojie, Jiang Rong, Wu Xia, Wang Jie, Wang Ying, Cao Xiyue, Xia Jianfei, Shi He, Tan Weiqiang
Department of Organ Transplantation, School of Medicine, Organ Transplantation Clinical Medical Center of Xiamen University, Xiang'an Hospital of Xiamen University, Xiamen University, Xiamen, 361102, People's Republic of China.
College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Instrumental Analysis Center of Qingdao University, Qingdao University, Qingdao, 266071, People's Republic of China.
Sci Rep. 2025 Feb 12;15(1):5235. doi: 10.1038/s41598-025-89688-5.
Herein, the coordination bond connected porphyrin-MOFs/MXenes composites has been prepared to construct an electrochemical sensing system for in situ and real-time monitoring of HO released by cells. The composites were synthesized by introducing 4-mercaptopyridine and utilizing its binding interaction with titanium in Mxenes and iron in MOFs. This composite was then transferred to the surface of ITO to construct an electrochemical sensing system. The unique properties of Mxenes and porphyrin MOFs endowed the sensing system with excellent electrocatalytic activity, good electrical conductivity and desirable biocompatibility. The electrochemical detections of hydroquinone verified the superior electrochemical performances of the sensing system. The constructed system achieved sensitive electrochemical detection of HO with a detection limit of 3.1 µM and a linear range of 10 µM to 3 mM. Furthermore, the excellent biocompatibility of the composites ensured HeLa cells growth and proliferation on its surface. Based on these favorable properties, the sensing system successfully achieved in-situ and real-time monitoring of HO released by HeLa cells.
在此，通过配位键连接的卟啉 - 金属有机框架材料/二维过渡金属碳化物和氮化物复合材料已被制备出来，用于构建一个电化学传感系统，以原位实时监测细胞释放的过氧化氢。该复合材料是通过引入4 - 巯基吡啶，并利用其与二维过渡金属碳化物中的钛以及金属有机框架材料中的铁的结合相互作用合成的。然后将这种复合材料转移到氧化铟锡表面，构建一个电化学传感系统。二维过渡金属碳化物和卟啉金属有机框架材料的独特性质赋予了该传感系统优异的电催化活性、良好的导电性和理想的生物相容性。对苯二酚的电化学检测验证了该传感系统卓越的电化学性能。构建的系统实现了对过氧化氢的灵敏电化学检测，检测限为3.1 μM，线性范围为10 μM至3 mM。此外，复合材料优异的生物相容性确保了宫颈癌细胞在其表面生长和增殖。基于这些良好的特性，该传感系统成功实现了对宫颈癌细胞释放的过氧化氢的原位实时监测。
