Tacket C O, Maneval D R, Levine M M
Infect Immun. 1987 May;55(5):1063-9. doi: 10.1128/iai.55.5.1063-1069.1987.
The ability to colonize the small intestine is essential for the pathogenesis of diarrhea caused by enterotoxigenic Escherichia coli (ETEC). Colonization is mediated by fimbriae (pili), of which there are several antigenically distinct types, including colonization factor antigen I, colonization factor antigen II (CS1, CS2, and CS3), and PCF8775 (CS4, CS5, and CS6). These fimbriae are associated with certain ETEC O serogroups. Serogroup O159 has had no known colonization factor. We found a distinct plasmid-encoded fimbria composed of 19-kilodalton protein subunits associated with ETEC serotype O159:H4. Rabbit antibody against this purified fimbria reacted with a single 19-kilodalton protein band as seen by Western immunoblot of sheared-cell preparations. The rabbit antibody, treated with colloidal-gold-labeled goat anti-rabbit immunoglobulin G, bound specifically to fimbriae when cells were examined with an electron microscope. Of 10 available ETEC O159:H4 strains from Europe, Bangladesh, and Kenya, 6 expressed this type of fimbria; its true prevalence among ETEC strains is unknown. This putative colonization factor of O159:H4 joins other ETEC fimbriae as potentially useful immunogens against human diarrhea.
对小肠进行定植的能力对于产肠毒素大肠杆菌（ETEC）引起的腹泻发病机制至关重要。定植由菌毛介导，菌毛有几种抗原性不同的类型，包括定植因子抗原I、定植因子抗原II（CS1、CS2和CS3）以及PCF8775（CS4、CS5和CS6）。这些菌毛与某些ETEC O血清型相关。血清型O159尚无已知的定植因子。我们发现了一种由19千道尔顿蛋白质亚基组成的独特的质粒编码菌毛，它与ETEC血清型O159:H4相关。针对这种纯化菌毛的兔抗体与剪切细胞制剂的Western免疫印迹中出现的单一19千道尔顿蛋白条带发生反应。用胶体金标记的山羊抗兔免疫球蛋白G处理的兔抗体，在用电镜检查细胞时能特异性结合菌毛。在来自欧洲、孟加拉国和肯尼亚的10株可用的ETEC O159:H4菌株中，有6株表达这种类型的菌毛；其在ETEC菌株中的真实流行率尚不清楚。这种假定的O159:H4定植因子与其他ETEC菌毛一样，作为针对人类腹泻的潜在有用免疫原。
